SHR大鼠左室肥厚与室性心律失常发生的相关性研究  

The correlation between the hypertrophic ventricular tissue of SHR rats and ventricular arrhythmia occurred

作　　者：黄颖[1] 伍伟锋[2] 

机构地区：[1]广西壮族自治区民族医院心血管内科二病区,广西南宁530001 [2]广西医科大学第一附属医院心血管病研究所,广西南宁530021

出　　处：《重庆医学》2014年第14期1690-1692,共3页Chongqing medicine

摘　　要：目的探讨有/无左室肥厚(left ventricular hypertrophy,LVH)的自发性高血压(SHR)大鼠诱发室性心律失常(VA)的敏感性差异和意义。方法实验分3组,每组12只,LVH组为已出现明显高血压和LVH的16周龄SHR雄性大鼠,以出现血压升高但未发生LVH的8周龄SHR雄性大鼠作为非LVH对照组,并以16周龄的Wistar雄性大鼠作为空白对照组(12只);应用Langendorff低钾液灌注3组大鼠离体心脏并全程记录心电图,比较各组心律失常诱发率和心律失常评分的差异;苏木素-伊红(HE)染色和胶原纤维三色染色法(Masson)染色观察左室心肌组织病理学改变和胶原容积分数(CVF)。结果与非LVH对照组和空白对照组比较,LVH组开始发生心律失常的时间更早;LVH组室性心动过速(VT)、短暂室颤(TVF)和持续性室颤(SVF)的发生率、心律失常评分和CVF改变均显著大于非LVH对照组和空白对照组(P<0.05),心肌细胞明显肥大,细胞间质增多。结论伴有LVH的SHR大鼠VA发生率和严重程度明显增加,提示LVH与心律失常的发生密切相关。Objective To explore the susceptibility differences of ventricular arrhythmia （VA ） in SHR rats with/without left ventricular hypertrophy（LVH） and the significance .Methods The experiments were performed on isolated hearts of 8‐week（the non‐LVH control group ,n=12） ,16 week‐old SHR（the LVH group ,n=12） and age‐matched Wistar Kyoto rats（Wistar ,the blank control group ,n=12） ,which were perfused in Langendorff mode with oxygenated Krebs‐Henseleit solution followed by a K＋‐defi‐cient solution .The epicardial electrocardiogram was continuously monitored during all experiments .HE staining and collagen vol‐ume fraction（CVF）was used to evaluate the condition of LVH .Results Compared with the non‐LVH control group and the blank control group ,the low K＋ induced ventricular arrhythmia occurred earlier with increased incidences and duration in the hearts of the LVH Group ,the incidences of ventricular tachycardia（VT） ,transient ventricular fibrillation（TVF） ,sustained ventricular fibrillation （SVF） and CVF were higher in the hearts of the LVH Group（P〈0 .05） ,myocardial cell hypertrophy and myocardial cells intersti‐tial increased .Conclusion The ventricular arrhythmia occurred earlier with increased incidences and duration in the LVH tissue of SHR rats ,which implies that LVH is associated with VA .

关 键 词：心律失常 心性 SHR大鼠 左室肥厚 LANGENDORFF 

分 类 号：R541.7[医药卫生—心血管疾病]