健脾化湿颗粒对实验性IBS-D大鼠结肠黏膜5-HT表达的影响  

Effect of JPHSKL on the quantity of 5-Hydroxytryptamine of colonic mucosa of diarrhea-predominant irritable bowel syndrome rats

作　　者：赵立波[1] 王迎寒 刘玉玲 张晓峰 董岫岩[3] 郭洪杰[3] 赵淑艳[1] 

机构地区：[1]河北承德护理职业学院涉外护理系,河北承德067000 [2]河北省中药研究与开发重点实验室,河北承德067000 [3]承德医学院附属肛肠医院中医内科,河北承德067000

出　　处：《重庆医学》2014年第14期1713-1715,共3页Chongqing medicine

基　　金：2010年河北省科技支撑计划基金资助项目(10276105D-45);2013年河北省科技支撑基金资助项目(13277797D)

摘　　要：目的观察健脾化湿颗粒对腹泻型肠易激综合征(IBS-D)大鼠结肠黏膜5-羟色胺(5-HT)表达的影响。方法 60只清洁级Wistar大鼠,分为6组,每组10只。采用番泻叶灌胃配合束缚应激造模。造模成功后,健脾化湿颗粒低、中、高剂量组给药浓度分别为1.5、2.5、4.0g/mL(生药),得舒特组给药浓度3mg/mL灌胃给药。每日1次,连续2周,另外两组为不经任何处理的正常组和造模成功的模型组。实验结束免疫组织化学观察结肠标本5-HT水平的变化。结果 IBS-D模型大鼠结肠组织5-HT的水平显著高于正常组(P<0.01);除健脾化湿低剂量组外,各治疗组大鼠结肠组织中5-HT的水平显著低于模型组(P<0.01)。其中,健脾化湿颗粒高剂量组效果明显,与得舒特组差异无统计学意义(P>0.05)。结论健脾化湿颗粒可抑制IBS-D模型大鼠结肠黏膜5-HT过高表达,明显改善IBS-D模型大鼠胃肠功能。Objective To observe the effect of Jian Pi Hua Shi Ke Li（JPHSKL） on IBS‐D ,gastrointestinal peptide hormones of rats 5‐HT ,and explore the therapeutic mechanism .Methods 60 clean‐level Wistar rats were used in the experiment .The rats were randomly divided into normal group ,model group ,JPHS low ,middle ,high and Deshute group（10 rats in each group） .Used gavage senna and restraint stress to establish D‐IBS rat model .The drug contrations of JPHSKL low ,midle ,high group were 1 .5 ,2 .5 ,4 .0 g/mL ,3 mg/mol in Deshute group .Once each day ,for 2 weeks ,the other groups were nomol group without any treatment and mod‐el group which building successful .Immunohistochemical SABC method to observe 5‐HT level of colonic specimens .Results The 5‐HT level in colonic tissue of the rats in IBS‐D model were obviously higher than the nomal group（P〈0 .01） ,and the 5‐HT level in colonic tissue in JPHSKL treated group were obviously lower than the mold group （P〈0 .05） .Among them ,the JPHSKL high group had significant effect and there was no obviously difference with Deshute group （P〉0 .05） .Conclusion JPHSKL can im‐prove gastrointestinal function of IBS‐D model rats ,effect mechanism is relating to decrease the level of 5‐HT in colonic mucosa .

关 键 词：肠易激综合征 血清素 健脾化湿颗粒 5-HT 

分 类 号：R285.5[医药卫生—中药学]