柱花草茎段组织培养研究  

Studies on Stylosanthes Stem Culture

作　　者：单国燕[1,2] 郑金龙[1,2] 陈河龙[1,2,3] 高建明[1,2,3] 张世清[1,2,3] 易克贤[1,2] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南海口571101 [2]农业部热带作物生物技术重点开放实验室,海南海口571101 [3]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《热带农业科学》2014年第3期21-26,共6页Chinese Journal of Tropical Agriculture

基　　金：国家自然科学基金-柱花草炭疽菌致病相关基因的克隆及功能分析(No.31072076);热带牧草病害防控技术研究与示范(No.201303057);国家科技支撑计划"热带亚热带地区超高产牧草新品种选育及产业化示范"(No.2008BADB3B08);科技部国家科技成果重点推广计划项目"高蛋白热带豆科牧草配套养殖奶牛产业示范"(No.05EFN216900381)

摘　　要：以‘热研2号’柱花草茎段为外植体,研究不同激素种类及组合对试管苗诱导、增殖和生根的影响。结果表明:将带芽茎段接种在MS+6-BA 5.0 mg/L+GA30.5 mg/L的培养基中腋芽诱导率最高可达90%;最佳不定芽增殖培养基为MS+6-BA 1.0 mg/L+NAA 0.5 mg/L,其分化率达100%;最佳生根培养基为MS+IBA 1.0 mg/L+IAA1.0 mg/L。综上所述,该研究结果改变了丛生芽的产生途径,缩短了分化时间,提高了增殖速度,是快速繁殖柱花草苗的有效方法。In this experiment, the stems of Stylosanthes guianensis cv, Reyan2 were used as a material and the effects of hormones and their combinations on the propagation and rooting of the plantlets were also studied. The results showed that the highest frequency of shoot formation was obtained when explants（stem with buds） were cultured on MS＋6-BA 5.0 mg/L＋GA3 0.5 mg/L, the inducement rate of valid buds reached to 90%; The best adventitious bud proliferation medium was MS＋6-BA 1.0 mg/L＋NAA 0.5 mg/L and the differentiation rate was up to 100%;The best rooting medium was MS＋IBA 1.0 mg/L＋IAA 1.0 mg/L. This research changed the way of producing tufted multiple shoot, shortened the time of differentiation, and raised the speed of proliferation, It is the available way to quickly propagate the Stylosanthes.

关 键 词：柱花草 茎段 组织培养 

分 类 号：S541[农业科学—作物学]