黄芪用于阻断宫颈癌前病变机制的研究  

Mechanism of Blocking Cervical Precancerous Lesions with Astragalus Membranaceus

作　　者：朱建楠[1] 朱艳[2] 

机构地区：[1]辽宁医学院,辽宁省锦州市121000 [2]辽宁医学院第一附属医院

出　　处：《中国全科医学》2014年第13期1562-1564,共3页Chinese General Practice

摘　　要：目的探讨不同浓度的黄芪注射液在体外对宫颈永生化上皮细胞(H8)增殖抑制及凋亡作用的机制。方法四甲基偶氮唑盐微量酶反应比色法(MTT法)检测不同浓度(100、200、500、1 000 g/L)黄芪注射液对H8的体外增殖抑制作用。Annexin V-FITC/PI法检测经不同浓度黄芪注射液处理48 h后H8的凋亡率。Western blotting法检测凋亡相关蛋白Bcl-2、Bax、p53的表达。结果 MTT法检测结果显示,不同浓度的黄芪注射液对H8有明显的抑制作用,且呈时间和浓度的依赖性,各组细胞抑制率比较差异有统计学意义(F组间=4.782,F时间=5.539,P<0.05)。Annexin V-FITC/PI法检测结果显示,不同浓度的黄芪注射液均可导致细胞的凋亡。经黄芪注射液干预48 h后,凋亡率分别为空白对照组(4.492±1.084)%、100 g/L组(18.054±1.108)%、200 g/L组(29.368±1.115)%、500 g/L组(37.090±2.652)%、1 000 g/L组(54.614±3.052)%,各组间比较差异有统计学意义(F=3.782,P<0.05)。Western blotting法检测表明随着药物浓度的增加Bcl-2表达量逐渐减少,Bax、p53表达量逐渐增加。结论黄芪注射液对H8有较显著的抑制增殖和诱导凋亡作用。其机制可能与Bcl-2表达减弱、Box、p53表达增强有关。Objective To study the mechanism of different concentration of astragalus membranaceus injection on inhibiting the proliferation and apoptosis of cervical immortalized cells（ H8） in vitro. Methods MTT method was used to detect the effect of inhibiting proliferation of different concentrations of astragalus membranaceus injection（ 100,200,500 and 1 000 g / L） in vitro on H8 cells. AnnexinV-FITC / PI method was used to detect the apoptosis rate of H8 cells 48 hours after treated with different concentrations of astragalus membranaceus injction. Western blotting method was applied to detect the expression of apoptosis related protein Bcl-2,Bax and p53. Results The results of MTT method showed that different concentrations of astragalus membranaceus injection had significant inhibitory effect on H8 cells in vitro and the effect was dependent on time and concentration. The inhibition rate of each group showed statistically significant differences（ Finter-group= 4. 782,Ftime= 5. 539,P〈0. 05）. The results of Annexin V-FITC / PI method showed that different concentrations of astragalus membranaceus injection could all cause apoptosis. 48 h after the intervention of astragalus membranaceus injection,the apoptosis rate was（ 4. 492 ± 1. 084） % in the control group,（ 18. 054 ± 1. 108） % in the 100 g / L group,（ 29. 368 ± 1. 115） % in the 200 g / L group,（ 37. 090 ± 2. 652） % in the 500 g / L group and（ 54. 614 ± 3. 052） % in the 1 000 g / L group. The difference between each group was statistically significant（ F = 3. 782,P〈0. 05）. The results of Western blotting method showed that the increase of concentration could cause the decrease of the expression of Bcl-2 protein, and the increase of the expression of Bax and p53 protein. Conclusion The astragalus membranaceus injection can obviously inhibit and induce the apoptosis of H8 cells,and the mechanism may relate to the decreased expression of Bcl-2 protein and increased expression of Box and p53 protein.

关 键 词：黄芪 宫颈肿瘤 癌前状态 细胞增殖 细胞凋亡 

分 类 号：R711.74[医药卫生—妇产科学]