大黄素对腹腔严重感染大鼠血浆血小板活化因子和降钙素原及一氧化氮水平的影响  

Effect of Emodin on Plasma PAF,PCT and NO Levels in Rats with Severe Abdominal Infection

作　　者：高举[1] 刘弋[1] 

机构地区：[1]安徽医科大学第一附属医院普通外科,安徽省合肥市230022

出　　处：《中国全科医学》2014年第15期1795-1797,1810,共4页Chinese General Practice

摘　　要：目的探讨大黄素对腹腔严重感染大鼠血浆血小板活化因子(PAF)、降钙素原(PCT)、一氧化氮(NO)水平的影响。方法制备腹腔严重感染大鼠模型并将大鼠模型随机分成空白组、对照组及大黄素组,各12只,分别采用0.9%氯化钠溶液、四磨汤以及大黄素干预腹腔严重感染大鼠模型,采用酶联免疫吸附试验法检测大鼠PAF、PCT、NO水平的变化。结果治疗前,3组大鼠PAF、PCT、NO水平比较,差异均无统计学意义(P>0.05)。治疗后,3组大鼠PAF、PCT、NO水平比较,差异均有统计学意义(P<0.05);其中对照组、大黄素组PAF、PCT、NO水平较空白组降低,大黄素组PAF、PCT、NO水平较对照组降低(P<0.05)。结论大黄素可以明显降低腹腔严重感染大鼠的PAF、PCT、NO水平以此达到有效降低血浆内毒素水平,稳定体内自由基释放-清除机制及氧化-抗氧化机制,最终控制炎症反应,减轻腹腔感染,促进机体恢复。Objective To investigate the effect of emodin on plasma platelet activating factor（PAF）,procalcitonin（PCT） and nitric oxide（NO） levels in rats with severe abdominal infection.Methods The severe abdominal infection rat models were randomly divided into blank group,control group and emodin group,there were 12 rats in each group,and the three groups were respectively treated with 0.9% sodium chloride injection,Simo decoction and emodin.The levels of PAF,PCT and NO were detected by ELISA.Results There was no significant difference in levels of PAF,PCT and NO among three groups before treatment（P 〉0.05）.After treatment,there were significant differences in levels of PAF,PCT and NO among three groups（P〈0.05）.The levels of PAF,PCT and NO in control group and emodin group were significantly lower than those in blank group,levels of PAF,PCT and NO in emodin group were significantly lower than those in control group（P〈0.05）.Conclusion The increases in inflammatory mediators are positively correlated with the severity of abdominal infection.Emodin can effectively decrease levels of PAF,PCT and NO in rats with severe abdominal infection,so as to decrease the plasma endotoxin level,stabilize free radicals release- clearance mechanism and oxidation- antioxidation mechanism,control inflammation,relieve abdominal infection and promote the body＇s recovery.

关 键 词：大黄素 感染 大鼠 血小板活化因子 降钙素原 一氧化氮 

分 类 号：R63[医药卫生—外科学] R332[医药卫生—临床医学]