Use of Tetrazolium Salt INT for Estimation of Biological Activity of Activated Sludge Cultivated in SBR Process  

作　　者：Jian-Hui Wang Jun Yin Hai Lu Ying-Zi Lin 

机构地区：[1]School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China [2]Key Laboratory of Songliao Aquatic Environment, Ministry of Education, Jilin Jianzhu University, Changchun 130118, China

出　　处：《Journal of Harbin Institute of Technology(New Series)》2014年第2期97-102,共6页哈尔滨工业大学学报（英文版）

基　　金：Sponsored by the National Water Pollution Control and Management Technology Major Projects(Grant No.2012ZX07408001-07,2012ZX07201001-01)

摘　　要：The tetrazolium salt 2-（4-Iodophenyl） -3-（ 4-nitrophenyl ） -5-phenyltetrazolium chloride （INT） was used as a tool fi）r estimating the activity of the electron transport system （ETS） in activated sludge in a 40 L sequencing batch reactor （SBR） and domestie sewage as the organic substrate. The activity of INT-ETS during one SBR cycle, and the effeet of the ammonia concentration and the concentration of organic matter influent on the INT-ETS activity were investigated. The results show that： the use of INT is reliable in estimating of biological activity of activated sludge of SBR system; Biological activity of organic matter biodegradation, nitrification and denitrification process in SBR system reduce orderly. Obviously, INT-ETS activity reduces from 232.59 rny/（g · h） to 190. 65 rag/（ g ·h） at first and then decreases to 113.88 my/（ g · h） when influent concentration of COD and NH4＋-N is 300 my/L and 40 mg/L respectively. In addition, various influent Nitrogen （NH4＋-N are 14.5 mg/L and 42.0 my/L） and organic shock loading （COD are 293 mg/L and 685 my/L） experimenntions cure prove that operational conditions have no obvious effect on INT-ETS variation rule. However, the time of the appearance of feature points marking different reaction phase is influenced.The tetrazolium salt 2-( 4-Iodophenyl)-3-( 4-nitrophenyl)-5-phenyltetrazolium chloride( INT) was used as a tool for estimating the activity of the electron transport system( ETS) in activated sludge in a 40 L sequencing batch reactor( SBR) and domestic sewage as the organic substrate. The activity of INT-ETS during one SBR cycle,and the effect of the ammonia concentration and the concentration of organic matter influent on the INT-ETS activity were investigated. The results show that: the use of INT is reliable in estimating of biological activity of activated sludge of SBR system; Biological activity of organic matter biodegradation, nitrification and denitrification process in SBR system reduce orderly. Obviously,INT-ETS activity reduces from 232. 59 mg /( g·h) to 190. 65 mg /( g·h) at first and then decreases to 113. 88 mg /( g·h) when influent concentration of COD and NH4 +-N is 300 mg / L and 40 mg / L respectively. In addition,various influent Nitrogen( NH4 +-N are 14. 5 mg / L and 42. 0 mg / L) and organic shock loading( COD are 293 mg / L and 685 mg / L) experimentations cure prove that operational conditions have no obvious effect on INT-ETS variation rule. However,the time of the appearance of feature points marking different reaction phase is influenced.

关 键 词：ETs activity aetivaied sludge SBR ＇ tetrazoliurn salt 

分 类 号：X703.1[环境科学与工程—环境工程]