铁线莲愈伤组织及不定芽诱导试验  

Study on induction of callus and adventitions buds in Clematis L.

作　　者：吴荣[1] 樊国盛[1] 王锦[1] 林萍[1] 

机构地区：[1]西南林业大学园林学院,云南昆明650224

出　　处：《广东农业科学》2014年第9期48-50,F0003,共4页Guangdong Agricultural Sciences

基　　金：国家林业局"948"项目(2008-4-11);云南省教育厅科研基金(09Y0308);西南林业大学面上科研基金(2009M28);云南省高校园林植物与观赏园艺重点实验室项目

摘　　要：以铁线莲Gipsy Queen的茎节和茎段为外植体,在MS基本培养基中添加0、1.0、2.0 mg/L 6-BA和0、0.01、0.05 mg/L NAA进行初代诱导和继代增殖培养。结果表明:最适合诱导愈伤组织的培养基为MS+NAA 0.01 mg/L+6-BA2.0 mg/L,诱导率可达50.0%,诱导出的愈伤组织呈淡黄色疏松颗粒状;最适合诱导侧芽的培养基为MS+NAA 0.01mg/L+6-BA 1.0 mg/L,出芽率可达170%;最适增殖培养基为MS+NAA 0.01 mg/L+6-BA 2.0 mg/L,增殖倍数达3.39。Callus and adventitions bud of Gipsy Queen were induced with columella and stem of Gipsy Queen as explants and with the MS added different concentrations of 6-BA（0, 1.0, 2.0 mg/L） and NAA（0, 0.01, 0.05 mg/L） as nutrient medium. The results showed that the callus could be easily induced in MS＋NAA 0.01 mg/L＋6-BA 2.0 mg/L, the induction rate was 50.0%, and the induced callus was loose and granular appeared yellowish. The best lateral bud induced medium was MS＋NAA 0.01 mg/L＋6-BA 1.0 mg/L, and the budding rate was 170%. The best multiplied medium was MS＋NAA 0.01 mg/L＋6-BA 2.0 mg/L, and the proliferation times was up to 3.39.

关 键 词：诱导 愈伤组织 不定芽 铁线莲Gipsy QUEEN 

分 类 号：S682.29[农业科学—观赏园艺]