海南淮山茎腐病病原生物学特性研究  

Study on biological characteristics of dioscorea stem rot disease in Hainan

作　　者：赵志祥[1] 陈圆[1] 肖敏[1] 肖彤斌 陈绵才[1] 

机构地区：[1]海南省农科院农业环境与植物保护研究所/海南省植物病虫害防控重点实验室,海南海口571100

出　　处：《广东农业科学》2014年第9期95-98,共4页Guangdong Agricultural Sciences

基　　金：海南省科学事业费项目(12-214001-0004);"十二五"国家科技支撑计划项目(2012BAD19B06)

摘　　要：淮山茎腐病是近年来发生在海南淮山中的一种重要病害,对海南海口、临高等地的淮山生产造成严重危害。通过菌丝生长速率法测定了培养基、温度、酸碱度、光照对淮山茎腐病菌菌丝生长的影响,同时,测定了湿度和养分对孢子萌发的影响。结果表明：PDA培养基最适合菌丝的生长;菌丝能够生长的温度范围为10~35℃,最适温度为25℃,致死条件为50℃处理10 min或55℃处理5 min;最适pH值为7.0,菌丝能够生长的pH值范围为5~10;光照条件则以黑暗条件下最佳,而孢子萌发的最佳相对湿度为100%,溶液成分对孢子萌发的影响不大。Dioscorea stem rot disease is a serious disease infected dioscorea in Hainan recently. At present, it has been broken out in Haikou and Lingao of Hainan, and caused serious economic losses to the dioscorea production in Hainan. In order to understand its biological characteristics, and to provide references for prevention and control of this disease, the method of mycelium growth rate was used to determine the influence of medium, temperature, pH value and illumination on mycelium growth. Also, the influence of moisture and nutrient on spore germination was assayed. The results showed that the most suitable culture medium was PDA and optimum temperature was 25℃. The temperature of the mycelium growth ranged from 10 to 35℃. And lethal temperature was 50℃ for 10 min or 55℃ for 5 min. The optimal pH value was 7.0. The pH value of mycelium growth ranged from 5 to 10. Dim light conditions was the best lighting condition, and the best relative humidity was 100% for spore germination, solution composition had little effect on spore germination.

关 键 词：淮山茎腐病菌 菌丝生长 孢子萌发 

分 类 号：Q93-33[生物学—微生物学]