基于线粒体控制区序列的海南须鲫遗传多样性分析  

Genetic diversity of Carassioides cantonensis of Hainan island based on mitochondrial control region sequences analysis

作　　者：邓春兴[1] 周文漪 郜星晨 许忠能[1] 章群[1] 

机构地区：[1]暨南大学生命科学技术学院,广东广州510632

出　　处：《广东农业科学》2014年第9期151-154,共4页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(41071034);暨南大学科研创新基金(21611426)

摘　　要：测定了海南岛南渡江和万泉河2个水系33尾须鲫线粒体控制区5'端520 bp序列,共发现5个变异位点,其中简约信息位点4个,没有插入、缺失,转换颠换比为4,定义了5个单倍型。万泉河和南渡江群体的单倍型多样度分别为0.6762(±0.1006)与0.6993(±0.0435),核苷酸多样度分别为0.00249(±0.001849)与0.00260(±0.001887),二者均处于较低水平。中性检验结果显示Fu's Fs为正值且不显著(0.288,P>0.1),核苷酸不对称分布不呈明显单峰,表明海南须鲫历史上没有发生快速种群扩张,种群大小相对稳定。2个群体间没有显著遗传分化(Fst=0.03638,P>0.1),海南须鲫群体可作为一个管理保护单位。Five hundred and twenty bp nucleotide sequences in the 5＇ terminal part of the mtDNA D-loop control region of 33 Carassboides cantonensis from 2 populations（Nandujiang and Wanquanhe） of Hainan island were determined.5 mutation sites defined 5 haplotypes, of which 4 sites were parsimony-informatic, and no insertions and deletion was found, transition transversion ratio was 4. The haplotype diversity of Wanquanhe and NanduJiang group were 0.6762（±0.1006） and 0.6993（±0.0435）, respectively, and the nucleotide diversity were 0.00249（±0.001849） and 0.00260（±0.001887）,respectively, both at a relative low level. Neutral tests showed that the Fu＇s Fs wasn＇t significant negative（Fu＇s Fs=0.288,P0.1）, and mismatch analysis wasn＇t obvious unimodal, which suggested that Carassioides cantonensis in Hainan island might not have experienced rapid population expansion and they were demographically stable in the history. No significant genetic differentiation was found between the 2 groups（Fst =0.03638, P 0.1）, so they could be maintained as a single management protection unit.

关 键 词：须鲫 线粒体控制区 遗传变异 

分 类 号：Q346.5[生物学—遗传学]