广西壮族哮喘患者ADAM33基因多态性与肺功能及哮喘病情的相关性研究  

Association of ADAM33 Gene Polymorphisms with Lung Function and Asthma Severity in Guangxi Zhuang Population with Asthma

作　　者：梁斯巧[1] 邓静敏[1] 韦旋[1] 陈晓丽[1] 梁联哨 

机构地区：[1]广西医科大学第一附属医院呼吸内科,南宁市530021

出　　处：《广西医学》2014年第5期569-571,共3页Guangxi Medical Journal

基　　金：广西医药卫生科研课题(Z2008142)

摘　　要：目的探讨整合素-金属蛋白酶33(ADAM33)基因多态性与肺功能及哮喘病情的相关性。方法对纳入的87例哮喘患者进行肺功能检查及病情分级,采用聚合酶链反应-限制性片段长度多态(PCR-RFLP)方法进行基因分型,分析S2位点CC基因型、CG基因型,T2位点GG基因型、AG基因型的分布情况及其与肺功能指标FEV1%、FVC%和哮喘病情的相关性。结果 S2位点基因型构成与肺功能指标FEV1%、FVC%相关,突变杂合子CG基因型组的肺功能较野生型CC基因型组好(P<0.05);T2位点基因型构成与FEV1%、FVC%无相关性(P>0.05),S2与T2位点多态性与哮喘病情严重程度无相关性(P>0.05)。结论 ADAM33基因多态性与广西壮族哮喘患者肺功能相关,S2位点C→G突变可能对广西壮族哮喘患者的肺功能具有保护作用,但该基因多态性与哮喘病情严重程度不相关。Objective To explore the correlation of a disintegrin and metalloproteinase 33 （ ADAM33 ） gene polymorphisms with lung function and asthma severity in Guangxi Zhuang population with asthma .Methods Lung function tests were conducted and asthma severity was classified in 87 asthmatic patients .Genotypes were determined by polymerase chain restriction fragment length polymorphism （ PCR-RFLP） .To analyze the distributions of genotype CC,genotype CG at locus S2 and genotype GG,genotype AG at locus T2 as well as the correlation of the distributions with lung function indexes （ FEV1%, FVC%） and asthma severity .Results The S2 genotype distribution correlated with lung function indexes （FEV1%,FVC%）,the lung function of patients with heterozygous genotype CG was better than that of patients with wild-type genotype CC（P〈0.05）.The T2 genotype distribution didn′t correlate with FEV1%and FVC%（P〉0.05）.Both S2 and T2 polymorphisms didn′t correlate with asthma severity （P〉0.05）.Conclusion ADAM33 gene polymorphism is associated with lung function in Guangxi Zhuang population with asthma ,C→G mutation at locus S2 may have a protective effect on lung function in Guangxi Zhuang population with asthma .However , the polymorphism of ADAM33 gene is not associate with asthma severity .

关 键 词：哮喘 整合素-金属蛋白酶33 基因多态性 肺功能 

分 类 号：R256.12[医药卫生—中医内科学]