细粒棘球蚴重组蛋白14-3-3和Eg10免疫差异的初步研究  

Preliminary study on the difference in immune protection between Echinococcus granulosus recombinant 14-3-3 and recombinant Eg10

作　　者：王强[1] 王娅娜[1,2,3] 王程铖 王玉姣[1] 吕士玉 赵巍[1,2,3] 

机构地区：[1]宁夏医科大学包虫病实验室,银川750004 [2]宁夏医科大学医学遗传学与细胞生物学教研室,银川750004 [3]宁夏回族自治区遗传与生殖重点实验室,银川750004

出　　处：《四川大学学报（自然科学版）》2014年第3期592-596,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(81160203);宁夏医科大学校级课题(XM201018)

摘　　要：表达纯化细粒棘球蚴重组蛋白14-3-3(rEg14-3-3)和Eg10(rEg10),分别免疫小鼠,8w后进行细粒棘球蚴原头蚴攻击感染.计算免疫保护力,检测脾组织中CD4+T、CD8+T细胞亚群以及T细胞增殖情况.实验结果表明,rEg14-3-3免疫组小鼠具有85.3%抵抗细粒棘球绦虫感染的能力,而rEg10免疫组小鼠与对照组小鼠相比无免疫保护力;与PBS对照组相比,只有rEg 14-3-3免疫组的CD4+T细胞亚群的分布有明显差异,且rEg 14-3-3抗原能诱导淋巴细胞显著增殖.因此rEgl4-3-3蛋白能抑制细粒棘球蚴的生长,诱导小鼠产生高水平的免疫保护力,可作为疫苗候选分子.The ICR mice were immunized purified expression of recombinant proteins 14-3-3 （rEg14-3-3） and Egl0 （rEgl0） of Echinococcus Granulosus and boosted on the eighth weeks. Calculating the protec- tive immunity, CD4＋and CD8＋T lymphocytes subsets in splenic tissue were analyzed and the prolifera- tion of T cells was examined. Results showed that the mice immunized with rEg14-3-3 showed protective immunity of 85.3% against Echinococcus granulosus, whereas no protective immunity was detected in rEgl0 immunized group compared to the PBS control group. Compared with the control group, only CD4＋T cell subsets distribution of rEg 14-3-3 immunized group showed a statistic significance,recombi- nant 14-3-3 of E. Granulosus can stimulate T lymphocytes proliferation significantly. The above study concludes that recombinant 14-3-3 of E. Granulosus is capable of inhibiting the growth of Echinococcus granulosus protoscolices and inducing a high level of immunity in protection against protoscolices challenge in mice, and thus could be a potential candidate vaccine.

关 键 词：细粒棘球绦虫 rEgl4-3-3 rEg10 免疫保护力 

分 类 号：R392[医药卫生—免疫学]