细菌线性质粒的复制研究  

Research Progress of Replication of Linear Plasmid in Bacteria

作　　者：朱云霞[1] 赵昕[1] 张绮思 张海方[1] 

机构地区：[1]江苏大学基础医学与医学技术学院,镇江212013

出　　处：《生物技术通报》2014年第5期32-36,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31000076);中国博士后科学基金(2013M531278);江苏省博士后科研资助计划(1202011B)

摘　　要：细菌的线性质粒是近年来基础微生物学研究的一个新热点。线性质粒的复制与环状质粒相比,其最突出的特点就是它的5'末端填补机制——填补因为5'末端引物被切除所留下的缺口。根据线性质粒末端结构的不同将其分为两大类:一类具有共价闭合的发夹末端,另一类具有共价结合的末端蛋白。就细菌线性质粒复制的研究现状作一综述,重点介绍了具有两种不同末端结构的线性质粒5'末端填补机制。Linear plasmid is a new focus of basic microbiology research in recent years. The replication of linear plasmid, compared to the circular plasmid, has its own distinct characteristies-5＇end patching mechanism by which bacteria can fill the single-stranded gaps caused by the removal of RNA primers at 5＇end of the strand. According to the terminal structure of linear plasmid, linear plasmids can be divided into two categories ： one kind of terminal structure is covalently closed hairpin, the other one is the end capped with the terminal protein covalently. This review introduced the progress of study on the replication mechanism of linear play, mid and focused on the 5＇end patching mechanism of the two kinds of linear plasmid terminal structures.

关 键 词：线性质粒 复制 细菌 末端蛋白 发夹末端 

分 类 号：Q78[生物学—分子生物学]