转Bt cry1Ah基因抗虫玉米的分子检测及农艺性状分析  

Molecular Detection and Agronomic Traits Analysis of Insect-resistant Transgenic Maize Harboring Bt cry1Ah Gene

作　　者：戴军[1,2] 李秀影[2] 朱莉[2] 汪海[2] 张杰[3] 何康来[3] 郎志宏[2] 黄大昉[2] 

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621010 [2]中国农业科学院生物技术研究所,北京100081 [3]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193

出　　处：《生物技术通报》2014年第5期62-68,共7页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30970231);转基因生物新品种培育重大专项(2013ZX08003-001)

摘　　要：利用农杆菌介导法将具有自主知识产权的Bt cry1Ah基因转入玉米自交系综31中,在获得的1 764株转基因植株中筛选获得1株对玉米螟有显著抗性的转基因植株。对该抗性植株进行了室内和田间抗虫性分析及外源基因表达分析,并研究了其杂交后代的农艺性状。结果表明,该转基因植株对亚洲玉米螟田间抗虫性达到高抗水平;外源基因能够正常高效表达,在玉米抽穗期的叶片中Cry1Ah蛋白表达量最高,达到1μg/g鲜重;株高、穗位高、穗长、穗粗等与非转基因对照植株相比差异不大,秃尖长明显短于对照,千粒重及单株产量明显高于对照,这种差异在人工模拟虫害爆发情况下达到极显著水平。Bt cryIAh gene which is a novel gene cloned by Chinese scientist was introduced into inbred line zong31 via agrobacterium- mediated transformation. An insect-resistant transgenic event was acquired by population screening of 1 764 transgenic plants. In this report we evaluated the transgenic event＇s insect resistance through laboratory and field bioassay and analyzed the foreign gene expression and the agronomic traits of hybrids generations. The results indicated that the transgenic event had high resistance to Asian corn boner （ Ostrinia furnacalis ） , and it had no significant difference in the plant height, ear position, ear length and ear width between the transgenic event and non-transgenic plant. However, there were significantly shorter bald in transgenic event and thousand kernels weight and yield per plant were significantly higher than the control. It was extremely significantly difference in transgenic plant and non-transgenic plant under conditions of infestation by artificial simulation.

关 键 词：转基因玉米 种群筛选 分子检测 抗虫 农艺性状 

分 类 号：S513[农业科学—作物学]