玉米ZmCI-1B启动子及其7个5'端缺失体在转基因拟南芥中的活性分析  

Identification of ZmCI-1B Promoter and Its Seven Deletions in Transgenic Arabidopsis thaliana

作　　者：李业[1,2] 柳小庆[2] 李素贞[3] 周晓今[2] 杨文竹[2] 陈茹梅[2] 

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621010 [2]中国农业科学院生物技术研究所,北京100081 [3]河北农业大学农学院,保定071001

出　　处：《生物技术通报》2014年第5期69-75,共7页Biotechnology Bulletin

基　　金：转基因生物新品种培育科技重大专项(2013ZX08003-002)

摘　　要：将ZmCI-1B全长启动子及其7个5'端缺失的启动子片段的表达载体分别转化农杆菌GV3101,经PCR鉴定正确之后,以拟南芥为遗传转化受体,利用花序侵染法将各个表达载体转化拟南芥。用PCR法检测转化后的拟南芥植株,取阳性植株的幼苗或花、角果进行GUS组织化学染色。结果表明,ZmCI-1B启动子在拟南芥中的调控特性与玉米中的不同;ZmCI-1B启动子及其7个5'端缺失启动子转基因拟南芥植株中GUS的染色部位各异,说明了不同长度的启动子功能特性不同,推测其可能与启动子上的顺式作用元件有关。The expression vectors of ZmCI-IB promoter and its seven 5＇ truncated fragments were transformed into Agrobacterium tumefaciens strain GV3101. After identified by PCR assay, with Arabidopsis thaliana as genetic transformation, the expression vectors were transformed into Arabidopsis thaliana by floral-dip method. The transformed Arabidopsis thaliana plants were identified by PCR assay, then the seedings, flowers and siliques from positive plants were conducted to GUS histochemical staining. The results showed that the characterization of ZmCI-1B promoter in Arabidopsis thaliana is different from miaze. The ZmCI-1B promoter and its seven 5＇ truncated promoter-GUS constructs had different GUS staining in transformed Arabidopsis thaliana plants, revealing that different-length promoter had different promoting activity. The cis-acting elements on the promoter may contribute to this.

关 键 词：启动子5'端缺失片段 拟南芥 GUS染色 

分 类 号：Q943.2[生物学—植物学]