天目山野生春兰根中可培养内生细菌多样性研究  

Diversity of Culturable Endophytic Bacteria in the Roots of Cymbidium goeringii from Tianmu Mountain

作　　者：武永秀[1] 宋彤彤[1] 张瑞英[1] 刘亮[2] 邵红[1] 李潞滨[3] 孙磊[1] 

机构地区：[1]河北大学生命科学学院河北省微生物多样性研究与应用实验室,保定071002 [2]中国林业科学研究院林业研究所,北京100091 [3]浙江天目山国家级自然保护区管理局,临安311311

出　　处：《生物技术通报》2014年第5期170-173,共4页Biotechnology Bulletin

基　　金：国家自然科学青年基金项目(31100002);河北大学自然科学研究计划项目(2010-195)

摘　　要：为了解春兰植物内生细菌的多样性,采用稀释涂布法对表面灭菌的天目山野生春兰根内生细菌进行分离培养。通过R2A和TSA两种培养基共分离获得63株内生细菌。对16S rDNA序列测定结果进行系统发育分析可知,63株细菌分属于β-变形菌纲(31.74%)、γ-变形菌纲(7.94%)及厚壁菌门(60.32%)。其中厚壁菌门为最优势类群,芽孢杆菌属为最优势菌属,占分离总菌数的50.79%。天目山野生春兰根内生细菌多样性指数为1.56。结果表明,初春季节天目山野生春兰根内生细菌多样性较低。In order to investigate the diversity of endophytic bacteria in Cymbidium goeringii, the endophytic bacteria was isolated from the surface-sterilized roots of Cymbidium goeringii from Tianmu Mountain, Zhejiang Province. A total of 63 isolates were obtained from R2A and TSA plates. The 16S rDNA sequences of these isolates were determined and phylogenetic analyses revealed that 63 isolates belonged to β-proteobacteria （ 31.74% ） , γ-proteobacteria （ 7.94% ） and Firmicutes （ 60.32% ） . The dominant group was Firmicutes, and the dominant genera were Bacillus （ 50.97% ） . The Shannon-Weaver index of endophytic bacteria in the roots of Cymbidium goeringii from Tianmu Mountain was 1.56. The results showed that there is low species diversity of cuhurable endophytic bacteria in the roots of Cymbidium goeringii from Tianmu Mountain at the early spring season.

关 键 词：春兰 天目山 可培养内生细菌 多样性 

分 类 号：S682.31[农业科学—观赏园艺]