培养基种类对花生毛状根株系生物量和白藜芦醇含量的影响  

Effect of Biomass and Resveratrol Content of Peanut Hairy Root Strains in Different Media

作　　者：姚庆收[1] 姜吉刚[2] 武玉永[1] 梁乘榜[1] 

机构地区：[1]滨州医学院药学院基因工程教研室,烟台264003 [2]滨州医学院药学院化学教研室,烟台264003

出　　处：《生物技术通报》2014年第5期174-178,共5页Biotechnology Bulletin

基　　金：山东省自然科学基金项目(ZR2010HQ054);山东省高等学校青年骨干教师国内访问学者项目

摘　　要：旨在研究不同培养基对花生毛状根株系生物量和白藜芦醇含量的影响。利用发根农杆菌R1601侵染花生的叶片,诱导毛状根,利用PCR技术检测毛状根。使用5种培养基(1/2MS、MS、MS+500 mg/L羧苄西林钠、B5和N6)分别培养同一个株系的花生毛状根,4周后利用高效液相色谱法(HPLC)测量毛状根生物量和白藜芦醇含量。结果显示,不同培养基对花生毛状根株系生物量的积累有显著差异,1/2MS培养基和MS培养基有利于花生毛状根株系生物量的积累。不同培养基对花生毛状根株系中白藜芦醇的含量有显著差异,B5培养基和N6培养基有利于花生毛状根株系中白藜芦醇的积累。It was to study the effect of biomass and resveratrol content of peanut hairy root strains in different media. To induce hairy roots by using A.rhizogenes R1601 infect peanut leaves, and to detect hairy roots by PCR. Peanut hairy root strains were cultured with five kinds of media （ 1/2MS, MS, MS ＋500 mg/L carbenicillin sodium, B5, and N6 ） , the biomass and resveratrol content of hairy root strains were measured by HPLC after four weeks. Results showed that the accumulation of biomass and content of resveratrol of peanut hairy root strains had a significant effect in different kinds media, 1/2MS medium and MS medium were conducive for the biomass accumulation of peanut hairy root strains. B5 medium and N6 medium were conducive for the resveratrol accumulation of peanut hairy root strains.

关 键 词：发根农杆菌 花生 毛状根 白藜芦醇 生物量 

分 类 号：S565.2[农业科学—作物学]