反相高效液相色谱法测定人尿中多种多环芳烃代谢物  

The method for assaying major metabolites of hydroxyl polycyclic aromatic hydrocarbons in human urine by RP-HPLC-UV

作　　者：杨红梅[1,2] 王永生[2] 黎俊宏[3] 欧阳运富[3] 

机构地区：[1]江苏省常州市中心血站,江苏常州213004 [2]南华大学公共卫生学院,湖南衡阳421001 [3]江苏省常州市疾病预防控制中心,江苏常州213022

出　　处：《应用化工》2014年第5期957-961,共5页Applied Chemical Industry

基　　金：江苏省预防医学科研课题指导性项目(YZ201013);常州市科技局课题(CS20102018)

摘　　要：建立了用反相高效液相色谱-紫外检测法同时检测人尿中6种多环芳烃羟基代谢产物的新方法。采用 Dia-monsil TM C18（5μm，150 nm ×4.6 nm）色谱柱分离，以 V（甲醇）： V（乙酸钠缓冲液）=66：34为流动相，在波长280 nm检测，流速为0.95 mL/ min。结果表明，当9-羟基菲、α-萘酚、β-萘酚、1-羟基芘、2-羟基芴和9-羟基芴的摩尔浓度分别为0.105~500.0μmol/ L，0.106~135.0μmol/ L，0.120~135.0μmol/ L，0.085~50.00μmol/ L，0.094~200.0μmol/ L 和0.101~200.0μmol/ L 时，峰面积与质量浓度呈很好的线性关系，相对标准偏差分别为2.5％~4.4％，2.1％~4.3％，2.3％~3.2％，1.7％~3.9％，1.9％~2.6％和1.8％~3.5％（n =6），平均回收率分别为92.6％，100.4％，101.0％，91.0％，106.7％和95.5％。方法准确可靠，用于尿样测定，结果满意。A novel method for assaying major metabolites of 6 kinds of polycyclic aromatic hydrocarbons in human urine by RP-HPLC-UV were established. These were detected at 280 nm and separated on Dia-monsil TM C18（5 μm,150 nm × 4. 6 nm）by using sodium acetate buffer solution（66： 34,V： V）as mobile phase when the flow rate was 0. 95 mL/ min. The result showed that the calibration curves of 9-hydroxy-phenanthrene,α-naphthol,β-naphthol,1-hydroxypyrene,2-hydroxyfluorene and 9-hydroxyfluorene showed good linearities in the ranges of 0. 105 ~ 500. 0 μmol/ L,0. 106 ~ 135. 0 μmol/ L,0. 120 ~135. 0 μmol/ L,0. 085 ~ 50. 0 μmol/ L,0. 094 ~ 200. 0 μmol/ L and 0. 101 ~ 200. 0 μmol/ L,respective-ly. The relative standard deviations（RSD）were 2. 5% ~ 4. 4% ,2. 1% ~ 4. 3% ,2. 3% ~ 3. 2% ,1. 7% ~3. 9% ,1. 9% ~ 2. 6% ,1. 8% ~ 3. 5%（ n = 6）and the average recoveries were 92. 6% ,100. 4% , 101. 0% ,91. 0% ,106. 7% and 95. 5% ,respectively. The proposed method was accurate and suitable for the identification and quantification of these compositions in human urine.

关 键 词：反相高效液相色谱 多环芳烃 尿 

分 类 号：X52[环境科学与工程—环境工程]