青壮年猝死综合征脑干中缝背核5-羟色胺及5-羟色胺转运体免疫组化研究  

Immunohistochemical study on SMDS dorsal nucleus raphe 5-serotonin and 5-serotonin transporter

作　　者：吴华[1] 唐群[2] 瞿志雄[1] 王健[1] 王兴华[1] 

机构地区：[1]湖南省第二人民医院芙蓉司法鉴定中心,湖南长沙410007 [2]湖南中医药大学病理教研室,湖南长沙410208

出　　处：《中国现代医生》2014年第15期61-62,66,共3页China Modern Doctor

基　　金：湖南省教育厅科研项目(12C0259)

摘　　要：目的探讨5-羟色胺和5-羟色胺转运体在青壮年猝死综合征脑组织的表达及其意义。方法运用免疫组织化学S-P法检测10例正常脑组织和8例青壮年猝死综合征脑组织中5-羟色胺和5-羟色胺转运体的表达水平,利用图像分析技术测量阳性细胞的平均光密度值。结果青壮年猝死征死者脑干中缝背核5-羟色胺的平均光密度值低于正常脑组织,而5-羟色胺转运体的平均光密度值高于正常脑组织,差异均有统计学意义(P<0.05)。结论 5-羟色胺及5-羟色胺转运体的变化参与青壮年猝死综合征的发病过程,可作为诊断青壮年猝死综合征的参考指标。Objective To investigate the 5- serotonin and 5-serotonin transporter expression and significance in brain tissue of sudden manhood death syndrome. Methods Used immunohistochemical S-P staining method in 10 cases of normal brain tissues and 8 cases of sudden manhood death syndrome in expression of 5-HT and 5-serotonin transporter in the brain tissue, using image analysis technique to measure the mean optical density of positive cells of value, Re- sults The average optical density of the .5- serotonin in the dorsal nucleus raphe of sudden manhood death syndrome was lower than that in normal brain tissue, and the average optical density of 5-serotonin transporter was higher than that in normal brain tissue, the differences were statistically significant （P 〈 0.05）. Conclusion The changes of 5- HT and 5- serotonin transporter were involved in the pathogenesis of sudden manhood death syndrome, which can be used as a reference marker for diagnosis of sudden manhood death syndrome.

关 键 词：法医病理学 青壮年猝死综合征 5羟色胺 5羟色胺转运体 免疫组化 

分 类 号：R338[医药卫生—人体生理学]