益气补肾活血方对失用性骨质疏松模型大鼠BMD、BGP、BAP的影响  

Impacts of Yiqi Bushen Huoxue Formula on BMD,BGP and BAP in Disused Osteoporosis Model Rats

作　　者：于学美[1,2] 崔西泉[3] 董军[2] 唐迎雪[1] 

机构地区：[1]山东中医药大学,山东济南250355 [2]泰山医学院,山东泰安271016 [3]泰安市中医二院,山东泰安271000

出　　处：《世界中西医结合杂志》2014年第5期480-482,共3页World Journal of Integrated Traditional and Western Medicine

基　　金：山东省中医药管理局课题(No.2011-225)

摘　　要：目的观察益气补肾活血方对失用性骨质疏松大鼠骨密度(BMD)、大鼠骨钙素(BGP)、大鼠骨特异性碱性磷酸酶(BAP)等指标的影响。方法将假手术大鼠作为空白对照组,大鼠右侧股骨骨折后石膏固定的方法建立废用性骨质疏松模型,分为益气补肾活血方组、钙尔奇组、模型对照组。灌胃给药60 d后,骨密度仪检测BMD、骨矿盐(BMC),ELISA法检测血清中BGP、BAP的含量。结果益气补肾活血方组与钙尔奇组全身及股骨BMD及BMC均显著高于模型对照组(P<0.05),两组之间比较,差异无统计学意义(P>0.05);模型对照组血清BGP最低,与其他组比较差异有统计学意义(P<0.05);益气补肾活血方组与钙尔奇组均能降低血清中BAP含量,与模型对照组比较差异有统计学意义(P<0.05)。结论益气补肾活血方能够有效防止失用性骨质疏松模型大鼠的骨量丢失,并能有效抑制骨吸收的发生,促进骨形成。Objective To observe the impacts of Yiqi Bushen Huoxue Formula on bone mineral density（ BMD）,osteocalcin（ BGP） and bone- specific alkaline phosphatase（ BAP） in disused osteoporosis rats. Methods The pseudo- operation rats were selected in a blank control group. The plaster fixation was used after the right femoral fracture for the preparation of disused osteoporosis models that were divided into Yiqi Bushen Huoxue Formula group,Caltrate group and a model control group. In 60 days of medication,DXA was used to detect BMD and bone mineral salt（ BMC） and ELISA was used to detect serum BGP and BAP levels. Results In Chinese medicine group and western medicine group,the general and femoral BMD and BMC were all higher significantly than those in the model control group（ P〈0. 05） and the difference was not significant in comparison between the two groups（ P〉 0. 05）. In the model control group,serum BGP level was the lowest than the other groups,indicating the significant difference（ P〈0. 05）. Serum BAP was reduced in Chinese medicine group and western medicine group,and either of them was different significantly as compared with the model control group（ P〈0. 05）. Conclusion Yiqi Bushen Huoxue Formula effectively prevents from bone mass loss,inhibits bone absorption and promotes bone formation in the disused osteoporosis rats.

关 键 词：益气补肾活血方 失用性骨质疏松 骨密度 

分 类 号：R285.5[医药卫生—中药学] R681[医药卫生—中医学]