消痰和胃方对胃癌前病变大鼠IL-1β蛋白及mRNA表达的影响  

Effects of Xiaotan Hewei Formula on the Expressions of IL-1β and IL-1β mRNA in Rats with Precancerous Lesions of Gastric Cancer

作　　者：矫健鹏[1] 王丹[1] 魏品康[1] 

机构地区：[1]第二军医大学第二附属医院,上海200003

出　　处：《世界中西医结合杂志》2014年第5期483-486,516,共5页World Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金青年基金(No.81102565)

摘　　要：目的观察消痰和胃方对胃癌前病变大鼠胃黏膜组织中IL-1β蛋白及基因表达的影响。方法 Wistar大鼠50只随机分为正常组、模型组、验证组、维酶素组、中药组5组,每组10只。除正常组外其他4组采用高剂量N-甲基-N'硝基-N-亚硝基胍(MNNG)综合造模法干预32周建立大鼠胃癌前病变(PLGC)模型。验证组处死后取胃,验证造模成功,其余4组按照各自给药方法干预12周。Western Blot法检测4组大鼠胃黏膜组织中白细胞介素-1β(IL-1β)蛋白表达含量;RTPCR法检测4组大鼠胃黏膜组织中IL-1βmRNA表达含量。结果模型组、维酶素组IL-1β蛋白及IL-1βmRNA表达较正常组显著升高(P<0.05,P<0.01)。中药组IL-1β蛋白表达较模型组、维酶素组下降(P<0.05),与正常组相比差异无统计学意义(P>0.05);中药组IL-1βmRNA平均相对表达量较模型组显著降低(P<0.01)。结论消痰和胃方可下调胃癌前病变大鼠IL-1β蛋白及基因表达。Objective To observe the effects of Xiaotan Hewei Formula on the expressions of interleukin- 1β（ IL- 1β） and IL- 1β mRNA in rats with precancerous lesions of gastric cancer（ PLGC）. Methods Fifty Wistar rats were randomized into the normal group,model group,validation group,vitacoenzyme group and Chinese medicine group,with 10 in each. The N- methyl- N ＇- nitro- N- nitrosoguanidine（ MNNG） complex modeling method was used to establish the PLGC rat models in all groups except the normal group. The rats in the validation group were sacrificed to verify whether the modeling was successful. The other four groups were intervened with the corresponding medication for 12 weeks. The expressions of IL- 1β and IL- 1β mRNA were measured. Results Comparing with the normal group,the expressions of IL- 1β and IL- 1β mRNA were significantly increased in the model group and vitacoenzyme group（ P〈0. 05 or P〈0. 01）. Comparing with the model group and vitacoenzyme group,the expression of IL- 1β was significantly decreased in the Chinese medicine group（ P〈0. 05）. There was no significant difference between the normal group and Chinese medicine group in the expression of IL- 1β（ P〉 0. 05）. Comparing with the model group, the average relative expression of IL- 1β mRNA was significantly decreased in the Chinese medicine group（ P〈0. 01）. Conclusion Xiaotan Hewei Formula can down regulate the expressions of IL-1β and IL-1β mRNA in rats with PLGC.

关 键 词：消痰和胃方 胃癌前病变 IL-1Β 

分 类 号：R285.5[医药卫生—中药学] R735.2[医药卫生—中医学]