HPLC测定乐脉丸中赤芍的含量  

The content determination of radix paeoniae rubra in Lemai pill by HPLC

作　　者：王从敏 熊本立 徐小利 

机构地区：[1]重庆市铜梁县铜梁中医院,402560 [2]重庆市食品药品检验所重庆市药物过程与质量控制工程技术研究中心,401121

出　　处：《临床合理用药杂志》2014年第16期37-38,共2页Chinese Journal of Clinical Rational Drug Use

摘　　要：目的：建立乐脉丸中赤芍含量的HPLC测定方法。方法采用色谱柱为Waters Symmetry Shield RP18（5μm，3．9mm ＆#215;150mm）；流动相为乙腈-0．05％磷酸（13∶87）；检测波长为230nm。结果芍药苷的进样量在0．20403～2．04032μg （ r ＝0．9999）范围内与各自峰面积积分值呈良好线性关系，平均回收率分别为101．5％和100．3％，RSD分别为1．5％和1．4％（n＝6）。结论 HPLC测定乐脉丸中赤芍的含量方法简便、快速准确、灵敏度高，重复性好，可作为乐脉丸中赤芍的含量测定。Objective To establish the determination method of radix paeoniae rubra in Lemai Pills by HPLC meth-od.Methods The column was Waters Symmetry RP18（5um,3.9X150mm）;Acetonitrile-0.05% phosphoric acid（13：87）as mobile phase.Detection wavelength was 230nm.Results The injection volume of paeoniflorin ranged from 0.20403 to 2.04032（r=0.9999）,within the scope,with each peak,has a good linear relationship.The average respectively were 101.5%and 100.3%.RSD respectively were 1.5% and 1.4% （ n=6）.Conclusion The content determination of radix paeoniae rubra in Lemai pill by HPLC is handy ,fastly and accurately ,has high sensitivity and repeatability ,can be the determined meth-od of radix paeoniae rubra in Lemai pill .

关 键 词：乐脉丸 赤芍 HPLC含量测定 

分 类 号：R28[医药卫生—中药学] O657.72[医药卫生—中医学]