复方多肽核酸溶液对雏鸡免疫增强作用的研究  

Study on Immunological Enhancement of Compound Polypeptide Nucleic Acid Solution on Chicken

作　　者：张从敬 张再辉 刘霞 齐静[2] 孙振华 胡明[2] 刘玉庆[2] 骆延波[2] 

机构地区：[1]山东省健牧生物药业有限公司,山东济南250100 [2]山东省农业科学院畜牧兽医研究所,山东济南250100 [3]上海海利生物技术股份有限公司,上海奉贤201403

出　　处：《山东农业科学》2014年第5期115-117,共3页Shandong Agricultural Sciences

基　　金：山东省农业重大应用技术创新课题"超耐药菌在食物链中流行病学监测与防控技术研究";济南市历城区企业自主创新基金项目(编号:2011069);山东省科技发展计划项目"新型稠杂环类抗药性突变抑制剂的研制与应用"(编号:2012GGC02012);2013年山东省农业科技成果转化资金项目"禽病原菌抗药性监测与防控技术示范"

摘　　要：对7日龄健康雏鸡免疫前24 h按照分组分别使用复方多肽核酸溶液和生理盐水,然后以新城疫克隆30弱毒苗点眼滴鼻,在免疫后7、14天心脏采血,采用HI法检测新城疫抗体水平。结果表明:应用复方多肽核酸溶液及新城疫克隆30弱毒苗后各试验组7天抗体水平明显升高,14日龄、21日龄抗体水平经t检验,试验组与对照组组间差异显著(P<0.05),试验组间差异不显著(P>0.05)。14日龄时各试验组外周血T细胞值与对照组差异显著(t>1.96,P<0.05)。21日龄时各试验组外周血T细胞值与对照组差异极显著(t>2.57,P<0.01)。研究结果显示复方多肽核酸溶液是一种很有前途的生物免疫增强剂。The 7-day old healthy chicken were treated with compound polypeptide nucleic acid solution and normal saline respectively 24 hours before immunization.Then they were inoculated with attenuated vac-cine of Newcastle Disease Clone 30 through nostril and eye.After inoculation for 7 and 14 days,the heart blood samples were collected,the antibody levels were detected by hemagglutination inhibition test,and the T and B cell were counted byα-naphthyl acetate esterase （ANAE）method.The results showed that the anti-body levels of 14-and 21 -day old chicken in experimental group were obviously higher than those of con-trol.By t test,the differences between experimental group and control were significant （P〈0.05 ）,while those between experimental groups were not significant （P〉0.05）.The average peripheral blood T cell per-centage of A,B,C and D experimental groups of 14-day old chicken was significantly different from that of control（t〉1.96,P〈0.05）,and that of 21 -day old chicken was highly significantly different from that of control（t〉2.57,P〈0.01）.These results indicated that the compound polypeptide nucleic acid solution was a biological immune enhancer with good application prospect.

关 键 词：复方多肽核酸溶液 体液免疫 细胞免疫 α-醋酸萘酯酶法 抗体 T细胞比例 

分 类 号：S852.5[农业科学—基础兽医学]