益气养阴祛瘀方与性激素对NOD小鼠的整体疗效及免疫功能的干预作用  

Int ervention Effect of Yiqiyangyinquyu Formula Combined Sex Hormone on Overall Efficacy and Immunologic Function of Rats with Non-obese Diabetics

作　　者：黄绥心[1] 王新昌[2] 肖丽群[1] 吴启富[3] 范永升[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053 [2]浙江中医药大学附属第二医院,浙江杭州310005 [3]南方医科大学附属南方医院,广东广州510515

出　　处：《中国中医急症》2014年第5期788-791,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81173248)

摘　　要：目的观察益气养阴祛瘀方和性激素对非肥胖糖尿病(NOD)自发性干燥综合征(SS)样小鼠的影响。方法将60只NOD自发性干燥综合征(SS)样小鼠随机分为模型组、羟氯喹组、益气养阴祛瘀方组、脱氢表雄酮(DHEA)组、雌二醇(E2)组、脱氢表雄酮加雌二醇(DHEA+E2)组,每组10只。ICR小鼠10只为正常组。正常组和模型组给予生理盐水,羟氯喹组给予羟氯喹溶液75 mg/kg,中药组予益气养阴祛瘀方药液27.4 g/kg,DHEA组予DHEA混悬液37.5 mg/kg,E2组给予E2溶液0.375 mg/kg;DHEA+E2组给予DHEA 37.5 mg/kg,E20.375 mg/kg。观察各组小鼠饮水量、唾液分泌量的变化;测定其胸腺、脾脏和颌下腺质量并计算小鼠胸腺指数、脾脏指数和颌下腺指数。酶联免疫法检测血清中细胞因子TNF-α、IL-6水平。结果与模型组比较,各给药组NOD小鼠的饮水量和唾液分泌量均改善(P<0.05)。中药组和DHEA组NOD小鼠的胸腺、脾脏和颌下腺指数均改善(P<0.05)。中药组和DHEA组血清TNF-α、IL-6的水平(P<0.05)显著降低。结论益气养阴祛瘀方及DHEA在整体疗效和免疫功能等方面对NOD小鼠SS具有有效的治疗作用。Objective： To study the effect of Yiqiyangyinquyu Formula and sex hormone on rats with SS of NOD （Non-obese Diabetic）. Methods： 60 NOD rats were randomized into six groups with 10 in each： model group, hydroxychloroquine group, Yiqiyangyinquyu Formula group （TCM group）, DHEA group, E2 group, and DHEA＋E2 group. 10 ICR Rats were taken as normal control group. Rats of normal group and model group were given nomal saline. Hydroxychloroquine group was given hydroxychloroquine 75 mg/kg by gavage. Yiqiyangyinquyu Formula group was given 27.4 g/kg of Yiqiyangyinquyu Formula. DHEA group was given 37.5 mg/kg of DHEA. E2 group was given 0.375 mg/kg of E2. DHEA＋E2 group was given 37.875 mg/kg of DHEA and E2. The changes of water intake and saliva secretion were observed. Indices including thymus,spleen and submandibular weight were calculated. Serum cytokine levels of TNF-α and IL-6 were detected by ELISA. Results： Compared with the model group,water intake and saliva secretion of all the treatment groups improved （P〈0.05）. Thymus, spleen and submandibular indices of Yiqi yangyin quyu Formula group and DHEA group were found to be increased （P〈0.05） and serum levels of TNF-α and IL-6 to be reduced （P〈0.05 ）. Conclusion： Yiqiyangyinquyu Formula and D HEA have good effect on overall efficacy and immunologic function of rats with SS of NOD.

关 键 词：益气养阴祛瘀方 SS NOD小鼠 脱氢表雄酮 雌二醇 

分 类 号：R285.5[医药卫生—中药学]