平欧杂种榛遗传多样性的SSR分析  

SSR Analysis of Genetic Diversity of Ping'ou Hybrid Hazelnut

作　　者：贾建云[1] 陈新[1] 刘洪对[2] 张俊林 徐丽[1] 朱东姿 张力思[1] 刘庆忠[1] 

机构地区：[1]山东省果树研究所/山东省果树生物技术育种重点实验室,山东泰安271000 [2]山东省农业科学院,济南250100 [3]杭州市园林绿化股份有限公司,杭州310000

出　　处：《中国农学通报》2014年第13期25-28,共4页Chinese Agricultural Science Bulletin

基　　金：科技部科技基础性工作资助项目"国家核桃板栗种质资源平台的运行服务"(2013-048);农业部作物种质资源保护项目"核桃板栗种质资源收集;保存与利用"(2014NWB009);山东省农业良种工程"果树地方优势品种发掘与种质创新利用研究"鲁科农学(2011-086)

摘　　要：分析平欧杂种榛间的遗传关系,为遗传图谱的绘制及优良基因的选育奠定基础。该实验选用21份主栽品种,经DNA提取、SSR标记及NTSYSpc（version 2.11 a）对结果的分析建立遗传聚类图。筛选6对欧榛SSR引物对供试材料进行检测,共检测出71个遗传位点,全部为多态性遗传位点,多态位点百分率为100%。遗传相似系数为0.34-0.82,平均相似系数为0.55,当阈值为0.74时,供试样品可聚类分为4个组。C1、C13和C2聚为一组,即组Ⅰ;C6和C15聚为一组,即组Ⅱ;C7、C9、C14、C16和C10聚为一组,即组Ⅳ;其余样品聚为一组,即组Ⅲ。平欧杂种榛品种间具有丰富的遗传多样性,供试主栽品种间存在着复杂的亲缘关系;构建的技术体系可为其他相关试验提供参考。The analysis of genetic relationship of Ping＇ou hybrid hazelnut laid the foundation for the geneticmapping and the breeding of choiceness gene. 21 main cultivars were selected for the experiment. After DNAextraction, SSR molecular marker and NTSYSpc（version 2.11 a） analysis of the results, constructed adendrogram. 6 primer pairs of Corylus avellana were selected for the detection of the tested materials. 71genetic loci were detected, which were polymorphic loci in all. The percentage of polymorphic bands was100%. The SSR-derived genetic similarity was 0.34-0.82, with an average of 0.55. All the samples weredivided into 4 groups when the threshold value was 0.74. C1, C13 and C2 constituted the groupⅠ; C6 and C15constituted the groupⅡ; C7, C9, C14, C16 and C10 constituted the groupⅣ. The others constituted the groupⅢ. There were genetically abundant diversities among Ping＇ou hybrid hazelnut. The genetic relationships ofthe main cultivars were complex. Analysis system established in this study could offer reference for otherrelative researches.

关 键 词：平欧杂种榛 SSR 遗传多样性 

分 类 号：Q30[生物学—遗传学]