异育银鲫组织中γ-氨基丁酸A受体(GABA_AR)的免疫组织化学定位  

Immunohistochemical Localization of γ-aminobutyric Acid A Receptor(GABA_AR) in the Tissues of Carassius Auratus Gibelio

作　　者：周爱玲[1] 阮记明[1,2] 曹海鹏[1] 胡鲲[1] 王祎[1] 赵依妮[1] 杨先乐[1] 

机构地区：[1]上海海洋大学国家水生动物病原库,上海201306 [2]江西农业大学动物科学技术学院,南昌330045

出　　处：《中国农学通报》2014年第14期33-38,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"鱼类GABA受体鱼渔药安全性评价的研究"(31172430);科技部863计划项目"新型环境友好型渔药的研制与渔药安全性评价"(2011AA10A216);农业公益性行业专项"渔药使用风险评估及其控制技术研究与示范"(201203085)

摘　　要：观察γ-氨基丁酸A受体(GABAAR)异育银鲫中枢神经系统和外周组织中的特异性分布情况,探讨其在不同组织中的作用。本试验以GABAAR的α1亚基(GABAARα1)作为GABAAR的检测指标,采用免疫组织化学方法对异育银鲫端脑、中脑、小脑、延脑、肝脏、肾脏组织中的GABAAR进行定位研究。结果表明:在异育银鲫端脑、中脑、小脑、延脑、肝脏、肾脏中均有GABAAR存在,各组织中的GABAAR密度从高到低依次为:小脑、中脑、延脑、端脑、肾脏、肝脏。其中,小脑的分子层和蒲氏细胞层、中脑的视盖、延脑的迷走叶、端脑的纹状体、肾脏的肾小管以及肝细胞中GABAAR的密度较高。研究显示,GABAAR主要分布于异育银鲫的中枢神经系统,外周组织中也有少量分布。本研究旨在为异育银鲫GABAAR分布的定位研究奠定一定的科学基础。To evaluate the function of γ-aminobutyric acid A receptor （GABAAR） in different tissues by observing the specific distribution in central nervous system and peripheral tissues of Carassius auratus gibelio. Using GABAAR al subunit （GABAARα1） as the detection indicator of GABAAR, this study was conducted by immunohistochemical method to investigate the localization of GABAAR in the telencephalon, mesencephalon, cerebellum, medulla oblongata, liver and kidney of C. auratus gibelio. The results showed that, GABAAR widely existed in the telencephalon, mesencephalon, cerebellum, medulla oblongata, liver and kidney of C. auratus gibelio. Its highest density was recorded in the cerebellum, and followed by the mesencephalon, medulla oblongata, telencephalon, kidney and liver. Among these tissues, the density of GABAAR was high in the corpus striatum of telencephalon, the optic tectum of meseneephalon, molecular layer and Purkinje cells of cerebellum, the vagus lobe of medulla oblongata, kidney tubules and hepatocytes. The research revealed that GABAAR was mainly distributed in central nervous system of C. auratus gibelio, and also had a small amount of distribution in peripheral tissues. This study aimed at laying a scientific basis for the distribution and localization of GABAAR in C. auratus gibelio.

关 键 词：GABAAR 异育银鲫 免疫组织化学 定位 

分 类 号：S9[农业科学—水产科学]