长期腹腔注射氯胺酮大鼠肾脏和膀胱组织中组胺受体H1的表达及意义  

Significance of H1 receptor expression in kidney and bladder tissues of rats after long term ketamine intraperitoneal injection

作　　者：于永刚[1] 朱良振[1] 廖松柏[1] 刘刚[1] 于管天[1] 

机构地区：[1]解放军第一八一医院泌尿外科,桂林541002

出　　处：《中华泌尿外科杂志》2014年第5期368-372,共5页Chinese Journal of Urology

摘　　要：目的 探讨长期腹腔注射氯胺酮后大鼠肾脏和膀胱组织中组胺受体H1的表达及其临床意义. 方法 2012年5-12月选取SPF级雄性SD大鼠60只,2月龄,体质量（200± 10）g.随机数字表法分为A、B两组,每组30只.A组以氯胺酮100 mg/kg腹腔注射,隔日1次;B组以相应量生理盐水腹腔注射,隔日1次.每周称体质量1次,校正用药量.分别于用药2、4、6个月后,两组各随机抽取10只,记录2h排尿次数,然后放入代谢笼24h,收集尿液,检测尿液中钠、钾含量.心尖采血,检测血肌酐水平,然后处死.切取大鼠肾脏、膀胱,应用免疫组化S-P法,标记大鼠肾脏及膀胱组织中组胺受体H1,用Imagepro-plus6.0图像分析软件计算各组中H1受体的平均吸光度A值,作为H1受体的表达水平.比较两组大鼠的尿量,2h排尿次数,24h钠、钾排泄量,血肌酐浓度及H1受体平均吸光度A值的差异. 结果 用药6个月后,观察大鼠膀胱组织病理变化证实建模成功率为90％（9/10）.A、B组24h平均尿量分别为（15.9± 1.3）、（10.1±0.8） ml;2h平均排尿次数分别为（6.9±1.4）、（3.0±0.5）次,差异均有统计学意义（P＜0.05）.A、B组24 h尿钠含量分别为（1.7±0.1）、（1.0±0.1）mmol,尿钾含量分别为（1.1±0.1）、（2.6±0.1）mmol,组间比较差异均有统计学意义（P＜0.05）.A、B组血肌酐分别为（60.5±6.8）、（58.1 ±3.9） μmol/L,差异无统计学意义（P＞0.05）.A组用药2、4、6个月后,肾脏H1受体的表达水平分别为0.008±0.001、0.016±0.001、0.023±0.004;B组分别为0.003±0.001、0.004±0.002、0.003±0.001,两组比较差异均有统计学意义（P＜0.05）.A组用药2、4、6个月后,膀胱组织H1的表达水平分别为0.017±0.006、0.031±0.012、0.036±0.007;B组分别为0.015±0.007、0.016±0.005、0.016±0.004,两组比较差异均有统计学意义（P＜0.05）. 结论 长期腹腔注射氯胺酮可引起大鼠的膀胱和肾脏损害,其机制与H1受体过表达有关.大鼠Objective To investigate the expression and clinical significance of H1 receptor in kidney and bladder tissue of rats after long term ketamine intraperitoneal injection.Methods This study was conducted from May 2012 to December 2012.Sixty male 2-month-old SD rats,weighted （200±10） g,were randomly divided into Group A and Group B.Each group concluded 30 rats.In the Group A,Ketamine （100 mg/kg） was given as intraperitoneal injection every other day,while normal saline （100 mg/kg） was given in Group B.The dosage was adjusted every week according to the weight of rats.After 2,4 and 6 months,10 rats from each group were randomly chosen.First,the micturition number during 2 h was recorded.Then,urine samples over a 24 h period were collected and the content of Na＋ and K＋ were determined.Finally,the blood samples were obtained from the apex of heart for the creatinine determination.The kidneys and bladders were harvested after the rats were sacrificed.HE staining was conducted on all the tissues.Immunohistochemical S-P method was used to detect the expression of H1 receptor in the bladder and kidney tissues from Group A and Group B.The average optical density （A Value） in each group was separately calculated by Imagepro-plus 6.0 software.All the parameters,mentioned above,were carefully compared.Results The successive rate of establishing rats model was 90% （9/10）,according to the pathological result after 6 months injection.The urine volume of 24 h in group A and B were （15.9±1.3） and （10.1±0.8） ml,respectively.Micturition frequency during 2 hours in group A and B were （6.9±1.4） and （3.0±0.5） times.The urine volume of 24 h and micturition frequency during 2 hours were significantly increased in group A （P〈 0.05）.The urine sodium within 24 h in group A was （1.7±0.1） mmol,which is increased significantly than that in group B （1.0±0.1 mmol）.While the urine potassium was less in group A （1.1±0.1 mmol/d） than in group B （2.6±0.1 mmol/d） （P〈0.05）.But

关 键 词：氯胺酮 大鼠 泌尿系损害 组胺受体H1 

分 类 号：R692[医药卫生—泌尿科学]