一株红螺菌科光合细菌培养基的配比优化  

Optimization of medium for strains photosynthetic bacteria of Rhodospirillaceae

作　　者：李立欣[1] 罗克洁[1] 唐二葵[1] 马放[2] 

机构地区：[1]黑龙江科技大学环境与化工学院,哈尔滨150022 [2]哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150090

出　　处：《黑龙江科技学院学报》2014年第2期141-143,148,共4页Journal of Heilongjiang Institute of Science and Technology

基　　金："十二五"国家科技支撑计划项目(2012BAD14B06-04);黑龙江省教育厅科学技术研究项目(12513088)

摘　　要：为获得一种高效培养光合细菌L-01的方法,采用表征菌体密度的参数OD660作为评价指标,通过单因素实验和正交实验分析其对碳源、氮源、磷源和生长因子的利用能力。结果表明,乙酸钠为光合细菌L-01的最大影响因子,其优化培养基配方为:乙酸钠7 g/L、氯化铵0.25 g/L、磷酸氢二钾0.1 g/L、酵母膏0.2 g/L、氯化镁0.2 g/L和九水硫化钠0.3 g/L。该结果为高密度培养光合细菌提供了基础数据。This paper features a highly effective approach to culturing photosynthetic bacteria L- 01. The approach is developed by analyzing photosynthetic bacteria L- 01 ＇s capability of using carbon source,nitrogen source,phosphorus source,and growth factor,using as the evaluation index the parameter OD660characterizing the density of bacteria and by virtue of single factor experiment and orthogonal experiment analysis. The analysis finds that acetate sodium behaves as the biggest impact factor of photosynthetic bacteria L- 01,with the optimized culture medium ingredients composed of CH3COONa 7 g / L, NH4Cl 0. 25 g / L,KH2PO40. 1 g / L,yeast extract 0. 2 g / L,MgCl20. 2 g / L and NaS·9H2O. The finding may serve as the basic data for high-density culturing of photosynthetic bacteria.

关 键 词：光合细菌 培养基 优化 正交实验 

分 类 号：Q935[生物学—微生物学]