清热化痰法对AECOPD大鼠血清中性粒细泡弹性蛋白酶水平的影响研究  

Effect of Clearing Heat and Resolving Phlegm on Blood Level of Neutrophil Elastase in AECOPD Rats

作　　者：李杰[1] 王琦[2] 冯淬灵[1] 张立山[1] 邱泽计[2] 吴珺[3] 罗慧[1] 董凤兰 刘福鼎[1] 赵福成[1] 齐莹[1] 薛广伟[1] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]北京中医药大学东方医院 [3]北京中医药大学基础医学院 [4]北京昌平城区社区卫生服务中心

出　　处：《中国药师》2014年第5期722-724,共3页China Pharmacist

摘　　要：目的:初步探究清热化痰法指导下的蒌芩止嗽煎对慢性阻塞性肺病急性加重期(AECOPD)大鼠血清中性粒细胞弹性蛋白酶水平的影响。方法:建立大鼠AECOPD模型,60只AECOPD大鼠模型随机分为模型对照组(20只)、氨溴索组(20只)、蒌芩止嗽煎组(20只),用酶联免疫吸附法(ELISA)测定AECOPD大鼠模型治疗前后血清中中性粒细胞弹性蛋白酶(NE)指标。结果:蒌芩止嗽煎组、氨溴索组治疗后与治疗前比较,NE均有明显下降(P<0.01)。结论:清热化痰法指导下的蒌芩止嗽煎初步研究是通过降低NE水平,从而减少AECOPD大鼠模型的气道黏液高分泌,减轻气道阻塞。To preliminarily study the effect of Louqin Zhisou decoctions with the mechanism of clearing heat and resol- ving phlegm on the blood level of neutrophil elastase （NE） in the rat model of acute exacerbation of chronic obstructive pulmonary dis-ease （AECOPD）. Methods： The rat model of AECOPD was established by passive cigarette smoking, intratracheal instillation of li- popolysaeehriele （LPY） and intranasal instillation of staphylococcus aureus. Totally 60 AECOPD rats were randomly divided into 3 groups, the model control group （ n = 20）, ambrohexel group （ n = 20 ）, and Louqin Zhisou decoctions group （ n = 20 ）. NE was detec-ted by ELISA. Results： Compared with that before the treatment, NE in ambrohexel group and Louqin Zhisou decoctions group was de-creased significantly（ P 〈 0.01 ）. Conclusion： Clearing heat and resolving phlegm method probably can decrease NE by reducing air- way mucus hypersecretion and obstruction in AECOPD rats.

关 键 词：清热化痰法 慢性阻塞性肺病急性加重期大鼠模型 中性粒细泡弹性蛋白酶 

分 类 号：R285.5[医药卫生—中药学]