不同产地寮刁竹药材中丹皮酚的含量的测定  

Determination of Paeonol in Cynanchum Paniculatum from Different Habitats

作　　者：林善远 

机构地区：[1]广东省新兴中药学校,广东新兴527400

出　　处：《中国药师》2014年第5期871-872,共2页China Pharmacist

基　　金：广东省科技计划项目(编号:2009B060600009)

摘　　要：目的：对不同产地的寮刁竹药材中丹皮酚含量进行测定，为更好地评价寮刁竹药材的质量提供依据。方法：用高效液相色谱法采用Promosil C18色谱柱（200mm×4.6mm，5μm），以甲醇-水（55：45）为流动相，流速1．0ml·min^-1，检测波长为274nm，进样量为10μl。结果：丹皮酚在0．0494～0．7908mg·ml^-1范围内线性关系良好（r=0．9999），回收率为99．98％，RSD为0．17％，不同产地寮刁竹中。丹皮酚含量有明显差异。结论：该方法简便、准确、重复性好，可用于不同产地寮刁竹药材中丹皮酚含量的测定。To determine paeonol in Cynanchum paniculatum from different habitats by HPLC to provide better quality evaluation for Cynanchum paniculatum. Methods： A Promosil C18 column （200 mm×4.6 mm, 5 μm） was adopted, methanol-water （55：45） was used as the mobile phase, the flow rate was 1.0 ml·min^-1, the detection wavelength was at 274 nm, and the injection volume was 10μl Results： Paeonol showed a good linearity （ r = 0. 999 9 ） within the range of 0. 049 4-0. 790 8 mg·ml^-1, and the re- covery was 99.98% with RSD of 0.17%. The content of paeonol in Cynanchum paniculatum from different habitats showed significant difference. Conclusion： The method is simple, accurate and reproducible, which can be used to determine the content of paeonol in Cynanchum paniculatum from different habitats.

关 键 词：高效液相色谱法 寮刁竹 丹皮酚 

分 类 号：R927.2[医药卫生—药学]