豫西地区犬巴贝斯虫病及媒介流行病学特征  

Epidemiological Characteristics of Canine Babesiosis and Main Vector of Infection in West of Henan

作　　者：尚泽松[1] 张才[1] 张振芳[1] 爨淑楠 杨自军[1] 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003

出　　处：《河南科技大学学报（自然科学版）》2014年第4期73-76,8-9,共4页Journal of Henan University of Science And Technology:Natural Science

基　　金：国家自然科学基金项目(31001058);国家级大学生创新训练计划(201210464024);河南科技大学SRTP项目(2012238;2013249)

摘　　要：为研究河南省西部地区犬巴贝斯虫病的流行情况及媒介蜱虫中巴贝斯虫的携带率,分别运用血液涂片及聚合酶链式反应的方法对豫西地区2 960只犬和813只蜱虫进行抽样调查。运用体式显微镜对蜱进行形态学观察,将部分聚合酶链式反应阳性产物进行测序分析,鉴定虫株。研究结果显示:豫西地区有犬巴贝斯虫病发生,犬患病率为3.41%;聚合酶链式反应测序结果均为吉氏巴贝斯虫基因序列;蜱虫的形态学观察结果显示:蜱虫以镰形扇头蜱为主(52.4%),血红扇头蜱略少,蜱虫带虫率为2.33%。To investigate the prevalence of canine Babesiosis and the carrying rate of Babesia in ticks in the western regions of Henan province,2 960 dogs and 813 ticks were measured separately by polymerase chain reaction(PCR) and blood smear. Ticks identification was performed with stereomicroscope. Parts of the PCR products were analyzed by sequencing to implement the classification in Babesia. The results show that the dogs are infected by Babesia in west of Henan province,and the prevalence rate is 3. 41%. Sequencing reveals that the products of PCR are the specific gene fragments of Babesia gibsoni. The morphological types for ticks are Rhipicephalus haemaphysaloides(52. 4%) and the tick parasite rate is 2. 33%.

关 键 词：巴贝斯虫病 犬 蜱 聚合酶链式反应 

分 类 号：S855.9[农业科学—临床兽医学]