贡梨果实黑斑病病原菌鉴定及室内防治药剂筛选  

Identification of the Pathogen of Pear Black Spot and Indoor Screening of Fungicides

作　　者：周求根 李诚[2] 蒋军喜[2] 龚玲玲 陈燕玲 

机构地区：[1]江西省井冈山应用科技学校,吉安343009 [2]江西农业大学农学院 [3]江西省贵溪市林业局

出　　处：《中国南方果树》2014年第3期35-38,共4页South China Fruits

基　　金：江西省教育厅科学技术研究项目(GJJ13269)资助

摘　　要：通过病原菌形态特征、致病性测定、rDNA-ITS序列和EF1-α基因序列分析,确定贡梨黑斑病的病原菌为链格孢菌Alternaria alternata。在此基础上,采用菌丝生长速率法测定8种杀菌剂对该病原菌的毒力大小。结果表明,苯醚甲环唑、异菌脲、肟菌·戊唑醇和苯甲·丙环唑的抑菌效果很好,EC50分别为0.490 4μg/mL、0.509 1μg/mL、0.571 8μg/mL和0.913 8μg/mL。The pathogen causing black spot of pear was identified as Alternaria alternate by morphological characteristics,pathogenicity test,and sequence analysis of rDNA-ITS and EF1-α.Indoor toxicity of eight fungicides was tested by using mycelium growth rate method.The results showed that four fungicides Difenoconazole,Rovral,Trifloxystrobin·Tebuconazole and Difenoconazole· propiconazole had high inhibitory effects on Alternaria alternate,and the EC50value was 0.4904 μg/mL,0.5091μg/mL,0.5718μg/mL and 0.9138μg/mL,respectively.

关 键 词：梨黑斑病 ALTERNARIA ALTERNATA 毒力测定 EC50 

分 类 号：S436.612.14[农业科学—农业昆虫与害虫防治]