血细胞分析仪法及直接计数法和涂片染色法单核细胞分类计数的比较  

The comparison among blood cell analyzer method, mononuclear cell direct counting and smear staining method in mononuclear cell count

作　　者：朱玉龙[1] 俞惠宇 李早荣[3] 

机构地区：[1]浙江省海宁市人民医院检验科,314400 [2]浙江省海宁市第二人民医院检验科,314400 [3]解放军第一一七医院检验科,314400

出　　处：《中国医师进修杂志》2014年第15期32-34,共3页Chinese Journal of Postgraduates of Medicine

摘　　要：目的 探讨三种单核细胞计数方法的准确性和可信性.方法 随机选取血细胞分析仪白细胞分类单核细胞＜15％、15％～ 20％、＞20％的标本,混匀后吸取20μl置单核细胞直接计数稀释液内计数,并按常规涂片,经瑞-吉染色后用显微镜人工分类,然后对三种方法结果进行比较.另外收集单核细胞比例高、中、低值标本各1份,用上述三种方法各计数30次.结果 血细胞分析仪白细胞分类单核细胞15％～ 20％、＞20％的标本与其他两种方法结果相关性差.三种方法的重复性比较,以低、中值标本以方法1及方法2较好,高值标本以方法3为优.结论 对单核细胞≥15％的标本,尤其散点图异常时必须经方法3（即涂片染色显微镜分类）再检查确认.Objective To.investigate the accuracy and reliability of monocyte count of three methods.Methods The specimens classified by blood cell analyzer WBC monocytes 〈 15%,15%-20%,〉 20% were taken randomly,draw 20 μ l after mixing,put in monocyte direct count dilution and count,and underwent routine smear,stained by Swiss-Gibbs and manual classified by microscope,and then compared the results of the three methods.Collected one copy each of the high,medium and low proportion of monocytes specimens,and counted 30 times by the three counting method.Results Blood cell analyzer in WBC monocytes 15%-20%,〉 20% had poor correlation with the results of the other two methods.Repeatability comparison of three methods,medium and low sample were better with method 1 and 2,high value sample was good with method 3.Conclusion The specimens of monocytes ≥ 15%,especially scatter plot is abnormal,must be confirmed by microscopic examination.

关 键 词：血细胞 单核细胞 直接计数 显微镜分类 相关性 

分 类 号：R446.11[医药卫生—诊断学]