外源性IL-15、IL-21对HIV-1感染者T淋巴细胞增殖的影响  

Effects of exogenous interleukin-15 and interleukin-21 on T lymphocytes proliferation in HIV-1-infected individuals

作　　者：平岖[1,2] 姚细安[2] 李玉枝[1,2] 赵令斋[2] 唐漾波[1,2] 

机构地区：[1]广州医科大学研究生学院,广东广州510182 [2]广州市第八人民医院传染病研究所,广东广州510060

出　　处：《热带医学杂志》2014年第4期476-480,共5页Journal of Tropical Medicine

基　　金：广州市医药卫生科技一般引导项目(2009-YB-088)

摘　　要：目的探讨外源性细胞因子IL-15、IL-21对HIV-1感染者T淋巴细胞增殖的影响。方法研究对象为24例已接受高效抗逆转录病毒治疗(HAART)及25例未经HAART的HIV-1感染者(HAART组及HAART-nave组)。以HIV-1P24编码区的氨基酸序列人工合成的12个肽段组成的肽段库作为特异性抗原表位,另添加外源性细胞因子IL-15或者IL-21,对两组研究对象的外周血单个核细胞(PBMC)进行体外刺激培养,采用羧乙基锗倍半氧化物(CFSE)对PBMC进行示踪染色;流式细胞仪检测刺激后的淋巴细胞的增殖情况。结果 HAART-nave组和HAART组中CD4+及CD8+T细胞的增殖百分率,两组的组内比较可见,IL-21刺激的实验组(IL-21组)和IL-15刺激的实验组(IL-15组)均显著高于相应对照组(P均为0.000);HAART组CD8+T细胞增殖水平,IL-15组及IL-21组均明显高于HAART-nave组(Z=-2.394,P=0.017;Z=-2.130,P=0.033);HAART组与HAART-nave组中实验分组IL-15组及IL-21组CD4+细胞增殖情况比较差异均无统计学意义。结论外源性IL-15、IL-21均能促进HIV-1感染者HARRT治疗前及治疗后CD4+及CD8+T淋巴细胞的增殖;且两种因子对CD8+T淋巴细胞增殖的作用HAART治疗后大于HAART治疗前。Objective To investigate effects of exogenous interleukin-15 and interleukin-21 on T lymphocytes proliferation in HIV-1-infected individuals. Methods Lymphocytes in PBMC from 24 cases with highly active antiretroviral therapy （HAART） and 25 cases with HAART-naive of HIV-infected individuals were stimulated with 12 synthetic specific peptides of amino acid sequences encoding HIV-1 P24 region,and treated with exogenous cytokine IL-15 or IL-21. Carboxy-fluorescein diacetate, succinimidyl ester （CFSE） labeling and flow cytometry were used to track and determine the proliferation of the treated lymphocytes. Results Proliferation of both CD4^＋ and CD8^＋ were increased in the addition of exogenous IL-15 or IL-21 than corresponding controls with significant difference （P=0.000）. Moreover, Proliferation of CD8^＋ was greater in HAART group than that in HAART-naive group with significant difference （Z=-2.394 ,P=0.017 ;Z= -2.130,P=0.033） ,and the proliferation of CD4^＋ was not statistical significance between two groups. Conclusions Exogenous cytokines IL-15 and IL-21 can promote proliferation of CD4^＋ and CD8^＋ lymphoeytes in vitro on both of HIV-1- infected individuals with HAART and HAART-naive. Proliferation of CD8^＋ lymphocytes of HIV-1-infected individuals with HAART was greater than that of those with HAART-naive.

关 键 词：HIV-1 外源性 IL-15 IL-21 T淋巴细胞 

分 类 号：R512.91[医药卫生—内科学]