pEGFP/Ang-1转染BMSCs对高糖环境中RF/6A细胞的保护作用  

pEGFP /Ang-1 transfected BMSCs attenuates glucose-damaged RF /6A cells

作　　者：侯阳[1] 李福智[2] 左中夫[3] 刘学政[3] 

机构地区：[1]辽宁医学院科学实验中心,中国辽宁省锦州市121001 [2]辽宁医学院附属第三医院胸外科,中国辽宁省锦州市121001 [3]辽宁医学院解剖学教研室,中国辽宁省锦州市121001

出　　处：《国际眼科杂志》2014年第5期807-810,共4页International Eye Science

基　　金：国家自然科学基金青年基金(No.81300931)~~

摘　　要：目的:观察血管生成素-1/重组质粒(pEGFP/Ang-1)转染的大鼠骨髓间充质干细胞(BMSCs)对高浓度葡萄糖损伤猴脉络膜-视网膜内皮细胞(RF/6A)的保护作用。方法:以pEGFP/Ang-1转染BMSCs,倒置荧光显微镜下观察增强型绿色荧光蛋白的表达,再利用Transwell模型,将转染的BMSCs与RF/6A共培养于高浓度葡萄糖培养基中。3d后MTT法检测RF/6A活力,Western blot检测磷酸化蛋白激酶B(phosphorylated protein kinase B,P-PKB)的表达,从而探讨转染pEGFP/Ang-1的BMSCs对高浓度葡萄糖培养中的RF/6A的保护作用。结果:成功转染pEGFP/Ang-1的BMSCs可见增强型绿色荧光蛋白表达,与转染pEGFP/Ang-1的BMSCs共培养的RF/6A细胞活力及P-PKB的表达均高于未转染组(均P<0.01),与对照组无明显差别(均P>0.05)。结论:质粒pEGFP/Ang-1转染的BMSCs对高糖环境中的RF/6A具有保护作用,其机制可能与P-PKB表达上调有关。AIM：To investigate the protective effects of pEGFP/Ang-1 transfected BMSCs on RF/6A in condition of high glucose concentration. METHODS, BMSCs were transfected pEGFP/Ang-1, confirming by observation of enhanced green fluorescent protein （EGFP） with fluorescence microscope. Rhesus chorioido-retinal endothelial cells （RF/6A） were cultured with or without pEGFP/Ang- 1 transfected BMSCs in transwell under high glucose concentration. Three days after co-cultured, cell vitality and pPKB expression of RF/6A were detected with methyl thiazolyl tetrazolium （MTT） methods and Western blot respectively, RESULTS. EGFP was expressed in BMSCs successfully transfected with pEGFP/Ang- 1. RF/6A cocultured with pEGFP/Ang- 1 transfected BMSCs showed higher viability and pPKB expression compared with RF/6A cultured in condition of high glucose concentration cultured with or without untransfected BMSCs （ P〈 0.01 respectively）, showing no difference to control group （ P〉 0.05 respectively）. ~ CONCLUSION- pEGFP/Ang- 1 transfected BMSCs showed protective effects, may via PKB, on RF/6A in condition of high glucose concentration. KEYWORDS： angiopoietin-1 ; bone marrow mesenchymal stem cells; Rhesus choroidal-retinal vascular endothelial cell; high glucose concentration

关 键 词：血管生成素-1 骨髓间充质干细胞 脉络膜-视网膜内皮细胞 高浓度葡萄糖 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]