苦菊提取物对肝损伤的保护作用及其安全性致突变作用的研究  

Protection of CichoriumendiviaL.Extract from Liver Injury and Its Mutagenesis

作　　者：陈超杰[1] 史蕤 展丽娟[3] 魏金锋 靳洪涛 秦海林[5] 王爱平 

机构地区：[1]福建医科大学福建省新药安全性评价中心,福州350108 [2]中国医学科学院/北京协和医学院,新药安全评价研究中心,北京100050 [3]中国医学科学院/北京协和医学院,国家心血管病中心,阜外心血管病医院,中国牛津国际医学研究中心,心血管疾病国家重点实验室,北京100037 [4]北京协和建昊医药技术开发有限责任公司,北京100176 [5]中国医学科学院/北京协和医学院药物研究所,中草药物质基础与资源利用教育部重点实验室,北京100050

出　　处：《福建医科大学学报》2014年第1期7-13,共7页Journal of Fujian Medical University

基　　金："重大新药创制"科技重大专项课题(2013ZX09302302);国家自然基金青年科学基金项目(81302756);福建省自然科学基金青年科技人才创新项目(2013J05118);福建医科大学博士启动基金(2011bs003)

摘　　要：目的考察苦菊提取物对肝损伤的影响及其致突变作用。方法通过噻唑蓝(MTT)法和2,7-二氯二氢荧光素乙酰乙酸(DCFH-DA)荧光染色,检测苦菊提取物对2,2’-偶氮-双-(2-脒基丙烷)氯化二氢(AAPH)致HepG2细胞损伤的存活率以及胞内活性氧(ROS)水平的影响;建立四氯化碳(CCl4)致小鼠肝急性损伤模型,以观察苦菊提取物对急性肝损伤小鼠的肝组织病理变化和血清中丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)水平的影响;以鼠伤寒沙门氏菌回复突变(Ames)试验检测苦菊提取物的致突变作用。结果 AAPH处理HepG2细胞后,细胞存活率下降,胞内ROS水平上升,而加入不同浓度的苦菊提取物则可明显改善上述结果;苦菊提取物能改善CCl4致小鼠肝急性损伤的肝细胞坏死和炎症病变,并可降低血清中ALT和AST水平,呈剂量依赖趋势,提示苦菊提取物具有肝保护的作用;苦菊提取物对鼠伤寒沙门氏菌株无致基因突变作用。结论苦菊提取物具有良好的肝保护作用,并初步判断其毒性不明显,且无致突变作用。Objective To investigate the protective effect of Cichorium endivia L. extract （CEE） on injured liver in vitro and in vivo, and the mutagenesis of CEE. Methods The viability and levels of the reactive oxygen species （ROS） in H epG2 cells exposed to 2,2＇-Azobis （2-methylpropionamidine） dihy- drochloride （AAPH） was measured by MTT assay and DCFH-DA fluorescence dye assay, respectively. The carbon tetrachloride （CC14） induced acute liver injury model in mice was used to evaluate the hepatic histopathological change and the levels of serum ALT and AST. The mutagenesis of CEE was assessed by Ames test. Results Treatment of HepG2 cells with AAPH reduced the cell viability and increased intracellular ROS levels, those effects could be reversed by addition of CEE. The hepatocyte necrosis and inflammatory change in acute liver injury mice induced by CC14 were improved by administration of CEE, the ALT and AST levels in the mice serum were reduced in a dose-dependent manner, indicating that CEE had the capacity of hepatoprotection. CEE did not show mutagenic effect as detected in Ames test. Conclusion CEE effectively protects the liver from the injury with little toxicity and no mutagenicity.

关 键 词：菊花 植物提取物 肝 乙酸盐类 细胞 培养的 疾病模型 动物 

分 类 号：R285.5[医药卫生—中药学]