α-突触核蛋白和硝基化α-突触核蛋白在不同年龄段食蟹猴结肠中的表达  

Differential expressions of α-synuclein and nitrated α-synuclein in colonic in different ages of cynomolgus monkeys

作　　者：杨斌[1] 杨巍巍[2] 李昕[2] 刘承伟[1] 于顺[2] 

机构地区：[1]桂林医学院病理和病理生理学系,广西桂林541002 [2]首都医科大学宣武医院神经生物学研究室北京市老年病医疗研究中心分子诊断实验室教育部神经变性病重点实验室,北京100053

出　　处：《首都医科大学学报》2014年第2期214-218,共5页Journal of Capital Medical University

基　　金：国家自然科学基金(81071014);国家重点基础发展计划(973)计划(2011CB504101);国家科技支撑计划课题(2012BAI10B03);北京市自然科学基金(7102076);首都卫生发展科研专项课题(首发2011-1001-01)~~

摘　　要：目的研究α-突触核蛋白(α-synuclein,α-syn)及硝基化α-突触核蛋白(nitratedα-synuclein,n-syn)在不同年龄段食蟹猴结肠中的表达。方法免疫组织化学方法检测α-syn和n-syn在不同年龄段食蟹猴结肠中的表达。免疫荧光双标观察α-syn和n-syn的共定位。结果α-Syn和n-syn在食蟹猴结肠中存在共定位。α-Syn和n-syn在不同年龄段食蟹猴结肠中均有表达,但α-syn和n-syn在老年食蟹猴表达量最高,在青年食蟹猴表达量最低。结论α-Syn和n-syn在食蟹猴结肠中存在共定位,且在老年猴中表达最高。Objective To explore the expressions of α-synuclein（α-syn） and nitratedα-synuclein（ n-syn） in colonic in different ages of cynomolgus monkeys. Methods Immunohis-tochemistry was applied to measure the expressions of α-syn and n-syn in colonic in different ages of cynomolgus monkeys. Double immunofluorescent labeling was used to examine the co-localization of α-syn and n-syn. Results α-Syn and n-syn were colocalized in colonic of cynomolgus monkeys. α-syn and n-syn were expressed in colonic in different ages of cynomolgus monkeys. However,α-syn and n-syn expressions were the highest in colonic of old monkeys and the lowest in colonic of young monkeys. Conclusion α-Syn and n-syn are colocolized in colonic of cynomolgus monkeys and are the highest in colonic of old cynomolgus monkeys.

关 键 词：Α-突触核蛋白 硝基化α-突触核蛋白 食蟹猴 

分 类 号：R338[医药卫生—人体生理学]