中介素8—47对大鼠急性胰腺炎微循环障碍的影响  

The effect of intermedin8-47 on microcirculation disturbance of acute pancreatitis in rats

作　　者：辛振磊[1] 胡杰[1] 闫盛[1] 王玉文[1] 刘新奇[1] 张超[1] 李海峰[1] 苏磊[1] 董红霖[1] 

机构地区：[1]山西医科大学第二医院血管外科,太原030001

出　　处：《中华肝胆外科杂志》2014年第5期380-383,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：山西省科技攻关项目（201303130174）

摘　　要：目的建立大鼠急性胰腺炎（AP）模型，观察外源性中介素8-47（IMD8-47）干预前后胰腺内、外分泌部微循环血管内皮细胞的变化，探讨IMD8-47对AP大鼠胰腺内、外分泌部微循环的影响。方法皮下注射蛙皮素建立大鼠AP模型：（1）检测各组大鼠胰腺水肿程度及血清淀粉酶含量；（2）光镜下观察各组胰腺组织病理学改变；（3）透射电镜观察胰腺内、外分泌部微循环血管内皮细胞超微结构变化；（4）免疫组化法检测胰腺内、外分泌部微循环血管内皮型一氧化氮合酶（eNOS）及血管内皮钙粘蛋白（VE-cadherin）表达情况。结果（1）胰腺组织水肿程度、病理评分及淀粉酶检测：AP组较IMD8-47干预组差异有统计学意义（P=0．035）；（2）干预前后胰腺内、外分泌部毛细血管内皮细胞超微结构发生改变；（3）免疫组化显示：对照组、干预组及模型组胰腺毛细血管内膜eNOS和VE—eadherin的含量内、外分泌部均有改变，差异有统计学意义（P〈0．05）。结论外源性IMD8-47能够对胰腺组织起到保护作用，但对胰腺内、外分泌部的影响存在差异。Objective To establish rat model of acute pancreatitis and observe the influence of Intermedin8-47 （IMD8-47） on microcirculation endothelial cell in the exocrine and endocrine parts of pancreas. Methods Rat model of acute pancreatitis is established by subcutaneous injection of bombesin ： （ 1 ） Evaluation of pancreatic edema and detection of the serum amylase level ; （ 2 ） Pathology observation of pancreatic tissue by Light-microscopy ; （3） Observation of ultrastructure changes of microcirculation endothelial cell in the parts of exocrine and endocrine by transmission electron microscope （TEM） ; （4） Detection of the expression of eNOS and VE-cadherin in endothelial cells by immune histochemical method. Results （ 1 ） Compared with IMD8-47 intervention group, AP Group had more serious edema, higher level of serum amyl- ase and pathological score of pancreatic tissue. The difference between the two groups was statistically significant （P = 0. 035） ; （2） TEM showed that after exogenous IMD8-47 intervention, the ultrastructure of blood capillary endothelial cells in the parts of exocrine and endocrine changed significantly ; （ 3 ） Immunohistochemical section showed that the eNOS and VE - cadherin expressions was significantly different among Control group, IMD8-47 intervention group and AP model group （P 〈 0.05 ）. Conclusion Exogenous IMD8-47 may contribute to protecting pancreatic tissue by the different effects on pancreatic exocrine and endocrine parts.

关 键 词：急性胰腺炎 微循环障碍 中介素8-47 内皮型一氧化氮合酶 血管内皮钙黏蛋白 

分 类 号：R657.51[医药卫生—外科学]