32份辣椒种质遗传多样性的同工酶分析  

Genetic Diversity among 32 Germplasms of the Genus Capsicumby Isozyme Analysis

作　　者：隋益虎[1] 李敏[1] 胡能兵[1] 赵岩[1] 苗永美[1] 张子学[1] 

机构地区：[1]安徽科技学院生命科学学院,安徽凤阳233100

出　　处：《西北植物学报》2014年第5期908-914,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：安徽省教育厅自然科学重点项目(KJ2013A074);安徽科技学院稳定人才基金(ZRC2013349);国家星火计划(2012GA710029;2012GA710028)

摘　　要：基于叶片的过氧化物酶（POD）、超氧化物歧化酶（SOD）及花蕾的酯酶（EST）同工酶酶谱的聚丙烯酰胺凝胶电泳（PAGE）分析,对32份辣椒种质的遗传多样性进行了研究。结果表明：（1）共产生29种谱带,其中POD谱带13种234条,多态性位点比例（PPL）和相对迁移率（Rf）变化范围分别为50.0%~80.0%和0.05~0.69;SOD谱带8种140条,PPL、Rf分别为100.0%、0.38~0.88;EST谱带8种163条,PPL、Rf分别为50.0%~87.5%、0.16~0.89。（2）共发现多态位点26个,PPL、总基因频率（Pt）、等位基因平均数（A）、Nei基因多样性指数（He）、Shannon信息指数（SII）、平均遗传一致度（GI）和遗传距离（GD）分别为89.66%、0.578 7、1.896 6、0.272 2、0.415 2、0.736 6和0.314 3;32份辣椒材料的Jaccard遗传相似系数为0.348~0.905,并聚类为4个栽培种群。（3）4个栽培种群内基因多样度（HS）为0.143 6;种群间基因多样度（DST）、基因分化系数（GST）、GI和GD分别为0.149 4、0.51、0.784 4和0.246 6。（4）9份紫色辣椒种质共发现多态位点16个,PPL、Pt、A、He、SII、GI和GD分别为55.17%、0.611 1、1.551 7、0.194 1、0.291 9、0.781 6和0.249 4。研究认为,32份辣椒材料的总体、栽培种间和种内以及紫色材料间遗传分化程度大,为紫色辣椒杂交育种的亲本选配与绿色品种的遗传改良提供了参考依据。Based on analyzed zymograms of leaf peroxidase isozyme （POD）,superoxide dismutase （SOD） and bud esterase （EST） by polyacrylamide gel electrophoresis （PAGE）,we studied the genetic diversity of 32 Capsicum genus germplasms.The results showed that：（1）A total of 29 kinds of bands were produced,including 13 kinds of 234 POD bands,the proportion of polymorphic loci （PPL） and the range of relative mobility （Rf） were 50.0%~80.0% and 0.05~0.69;8 kinds of 140 SOD bands,PPL was 100.0%,and Rf was 0.38~0.88;8 kinds of 163 EST bands,PPL was 50.0%~87.5%,and Rf was 0.16~0.89.（2）26 polymorphic loci were found,and PPL,total gene frequency （Pt）,mean number of alleles （A）,Nei’s genetic diversity index （He）,Shannon’s information index （SII）,average of genetic identity （GI） and genetic distance （GD） were 89.66%,0.578 7,1.896 6,0.272 2,0.415 2,0.736 6 and 0.314 3,respectively;Jaccard genetic similarity coefficients were in range of 0.348~0.905,by which 32 materials were clustered into 4 cultivated populations/species.（3）Genetic diversity within species （HS） of 4 cultivated species was 0.143 6,and genetic diversity among species （DST）,gene differentiation coefficients among species （GST）,GI and GD were 0.149 4,0.51,0.784 4 and 0.246 6,respectively.（4）In 9 purple germplasms,16 polymorphic loci were found,and PPL,Pt,A,He,SII,GI and GD were 55.17%,0.611 1,1.551 7,0.194 1,0.291 9,0.781 6 and 0.249 4,respectively.In conclusion,high genetic differentiation degree among overall tested materials,or among cultivated species and within species,or among purple materials will provide a reference to selecting parents of purple capsicum crossbreeding and genetic improvement of the green variety.

关 键 词：辣椒 过氧化物酶 超氧化物歧化酶 酯酶 聚类 遗传多样性 

分 类 号：Q814.9[生物学—生物工程]