西洋参皂苷对缺血-再灌注诱导乳鼠心肌细胞凋亡及钙浓度的影响  

Effect of total saponin of Pannax quinquefolius on cardiomyocyte apoptosis induced by ischemia/reperfusion and calcium concentration in suckling rats

作　　者：刘哲[1] 张峰[2] 李丽阳[1] 姚晓颖[2] 武瑞[3] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319 [2]大庆油田总医院骨科,黑龙江大庆163311 [3]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国生物制品学杂志》2014年第5期661-663,670,共4页Chinese Journal of Biologicals

基　　金："黑龙江省青年科学基金"项目资助(41411046-1-11014/C1202)

摘　　要：目的探讨西洋参皂苷(Panax quinquefolius saponin,PQS)对缺血-再灌注(ischemia/reperfusion,I/R)诱导乳鼠心肌细胞凋亡及钙浓度的影响。方法将原代培养的心肌细胞分为正常对照组(不做任何处理)、I/R组(心肌细胞经缺氧、无血清孵育2 h,再复氧、复血清培养24、48 h,以模拟心肌I/R损伤)和西洋参总皂苷干预组(PQS+I/R,细胞再灌注时分别加入5、10、20、40μg/ml PQS),采用台盼蓝染色法检测各组心肌细胞的活性;流式细胞术检测心肌细胞的凋亡率;激光扫描共聚焦显微镜检测心肌细胞内游离钙的变化。结果与I/R组比较,10、20、40μg/ml PQS+I/R组心肌细胞活性显著增加(P<0.01),心肌细胞凋亡率显著降低(P<0.05);20μg/ml的PQS可降低心肌细胞的钙负载。结论 PQS可显著减少I/R诱导的心肌细胞凋亡,并减轻心肌细胞钙超载的发生,这可能是PQS抑制I/R心肌细胞凋亡的机制之一。Objective To investigate the effect of Pannax quinquefolius saponin（PQS） on cardiomyocyte apotosis induced by ischemia / reperfusion（I / R）and calcium concentration in suckling rats. Methods Primary culture of cardiomyocytes was divided into normal control,I / R and PQS ＋ I / R groups. The cardiomyocytes in normal control group were untreated,while those in I / R group were cultured in serum-free medium under hypoxic condition for 2 h then in serumcontaining medium under aerobic condition for 24 and 48 h to mimic I / R injury,and those in PQS ＋ I / R group were added with 5,10,20 and 40 μg / ml total saponin of PQS at time of reperfusion. The activities of cardiomyocytes in various groups were determined by trypan blue staining,while the apoptosis rates by flow cytometry,and the changes of free calcium by laser scanning cofocal microscopy. Results The activities of cardiomyocytes in 10,20 and 40 μg / ml PQS ＋ I / R groups were significantly higher（P〈0. 01）,while the apoptosis rate was significantly lower,than those in I / R group（P〈0. 05）. However,20 μg / ml PQS decreased the calcium load of cardiomyoc- ytes. Conclusion PQS decreased the apoptosis of cardiomyocytes induced by I / R as well as the occurrence of over-load of calcium in cardiomyocytes,which might be a mechanism of PQS in inhibiting the cardiomyocyte apoptosis induced by I / R.

关 键 词：西洋参皂苷 缺血-再灌注 细胞凋亡 钙负载 

分 类 号：R542.2[医药卫生—心血管疾病] R961.1[医药卫生—内科学]