胶束毛细管电泳测定女贞子饮片中6种指标成分的含量  

Simultaneous Determination of Six Index Components in Ligustri Lucidi Fructus by MEKC-DAD

作　　者：周逸芝[1] 邹立思[1] 刘毅[1] 刘训红[1] 韩乐[1] 李俊松[1] 蔡宝昌[1] 

机构地区：[1]南京中医药大学江苏省中药炮制重点实验室,南京210023

出　　处：《中国药学杂志》2014年第11期994-998,共5页Chinese Pharmaceutical Journal

基　　金：江苏省中药炮制重点实验室开放式课题(ZYPZ007);江苏省科技厅"科技基础实施建设计划"专项(BM2009903);江苏高校优势学科建设工程资助项目(ysxk-2010)

摘　　要：目的 建立胶束电动毛细管色谱二极管阵列检测法（MEKC-DAD）同时测定女贞子饮片中红景天苷、酪醇、女贞苷、特女贞苷、齐墩果酸和熊果酸含量的方法 。方法 60 mmol·L^-1硼砂-10 mmol·L^-1十二烷基硫酸钠（SDS）-30 mmol·L^-1羟丙基-β-环糊精-10%的甲醇（pH 9.03）为电泳介质,未涂渍标准熔融石英毛细管（75μm×64.5 cm,有效长度56 cm）为分离通道,分离电压为20 kV,检测波长为210 nm,毛细管温度为25℃,压力进样为5 kPa×6 s。结果 6种指标成分的浓度与峰面积的线性关系良好（r〉0.95）;加样回收率为94.57%-102.07%（RSD〈5%）;用此法测定了女贞子饮片中6种指标成分的含量,结果 满意。结论 该方法 准确、可靠,重复性较好,可用于女贞子饮片内在质量的评价和控制。OBJECTIVE To establish a micellar electrokinetic chromatography（MEKC） method for simultaneous determination of salidroside, tyrosol, ligustroflavone, specnuezhenide, oleanolic acid, and ursolic acid in Ligustri Lucidi Fruetus. METHODS 60 mmol · L^-1 borax-10mmol · L^-1 SDS-30 mmol · L^-1 hydroxypropyl-beta-cyclodextrin-10% methyl alcohol was used as the buffer solution （pH 9. 03 ）. Uncoated fused silica capillary （75 μm × 64. 5 cm, 56 cm of effective length） was used with separation voltage of 20 kV. The detection wavelength was set at 210 nm. The column temperature was maintained at 25 ℃ , and the sample was injected at 5 kPa × 6 s. RESULTS The calibration curves of the six index components showed good linearity （ r 〉 0. 95 ） in the range of the tested concentrations, and the average recoveries of the method were between 94. 57% and 102. 07% （RSD 〈 5% ）. CONCLUSION The method is simple, accurate and reproducible, and can be used for the quality control of Ligustri Lucidi Fructus.

关 键 词：胶束毛细管电泳法 女贞子 指标成分 含量测定 红景天苷 酪醇 女贞苷 特女贞苷 齐墩果酸 熊果酸 

分 类 号：R917[医药卫生—药物分析学]