高效液相色谱法测定氯霉素滴眼液中硫柳汞和苯基汞的含量  

Determination of Thimerosal and Phenylmercuric Compounds in Chloramphenicol Eye Drops by HPLC

作　　者：易大为[1] 马琳[1] 张亚杰[1] 

机构地区：[1]辽宁省食品药品检验所,沈阳110023

出　　处：《中国药学杂志》2014年第11期1004-1008,共5页Chinese Pharmaceutical Journal

摘　　要：目的 建立氯霉素滴眼液中硫柳汞和苯基汞防腐剂的含量测定方法 。方法 采用高效液相色谱法,色谱柱为Agilent苯基柱[Zorbax Eclipse XDB-phenyl（4.6 mm×250 mm,5μm）],0.05 mol·L^-1磷酸二氢钾溶液（用三乙胺调节pH值至5.5）-乙腈（82∶18）为流动相,流速为1.2 mL·min^-1,检测波长为220 nm。结果 硫柳汞和醋酸苯汞在2-100μg·mL^-1之间呈良好的线性关系（r〉0.999 0）,硫柳汞的平均回收率≥96.9%,醋酸苯汞的平均回收率≥99.1%。结论 本法简便、准确、专属性强,可用于药品中有机汞防腐剂的控制。OBJECTIVE To develop an HPLC method for simultaneous determination of thimerosal and phenylmercuric compounds in chloramphenicol eye drops. METHODS The analysis was performed on a phenyl-column with an ion-suppress mobile phase system consisting of 0. 05 mol · L^-1 potassium dihydrogen phosphate solution （pH adjusted to 5.5 with triethylamine）/acetonitrile （ 82： 18, V/V） at a flow rate of 1.2 mL · min^-1 at 220 nm. RESULTS The established method had good linearity within the concentration range of 2.0 - 100 μg ·mL^-1 （ r 〉 0. 999 0） for thimerosal and phenylmercuric acetate, with average recoveries of ≥96. 9% for thimerosal and ≥99. 1% for phenylmercuric acetate. CONCLUSION The method is simple, accurate and specific for determination of thimerosal and phenylmercuric compounds in chloramphenicol eye drops.

关 键 词：高效液相色谱法 苯基柱 硫柳汞 苯基汞化合物 氯霉素滴眼液 

分 类 号：R917[医药卫生—药物分析学]