他达拉非对大鼠脑缺血-再灌注损伤急性期的神经保护作用  

Neuroprotective effect of tadalafil on ischemia/reperfusion injury in rat brain

作　　者：韩建华[1] 丁钋[1] 张超[2] 

机构地区：[1]天津市人民医院医务处,天津300121 [2]天津医科大学,天津300070

出　　处：《中风与神经疾病杂志》2014年第5期412-414,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨他达拉非对急性期大鼠脑缺血-再灌注损伤的神经保护作用。方法采用夹闭双侧颈总动脉的方法 Wistar大鼠大脑缺血-再灌注模型。将30只Wistar大鼠随机分为3组(每组10只):对照组(control),再灌注组(IR)和他达拉非组(IR+TAD)。采用Garcia神经功能评分进行功能评定,TTC染色测量各组脑梗死面积,应用硫代巴比妥酸比色法检测各组丙二醛(MDA)浓度,以免疫荧光染色测定大脑梗死局部MAP2蛋白表达。结果他达拉非组再灌注后的梗死面积(1.95±2.11 mm2)、脑组织中MDA水平(8.99±0.21 nmol/g)小于再灌注组(3.78±1.87 mm2)、(12.22±0.55 nmol/g)(P<0.05),Garcia评分、脑组织微管结合蛋白(MAP)2蛋白高倍镜下单位视野密度(10.43%±7.19%)大于再灌注组(6.16%±5.22%)(P<0.05)。结论他达拉非可以降低羟自由基水平,对抗神经细胞凋亡,对脑缺血-再灌注损伤大鼠神经具有保护作用。Objective To determine the neuroprotective effect of tadalafil on short-term ischemia /reperfusion injury in rat brain. Methods The study was carried out on 30 Wistar-albino rats,which were divided into three groups including a control group（ n = 10）,an I / R group（ n = 10） and an I / R ＋ TAD group（ n = 10） [2 mg /（ kg·d） for 4 days before ischemia]. Garcia score was evaluated. After that,at the end of the experiment,tissue samples were collected for both biochemical and histopathological analyses： malondialdehyde level,infarct area,and microtubule-associated protein 2 density. Results The infract area evaluated in TAD-administered group（ 1. 95 ± 2. 11 mm2） was significantly lower than that in IR group（ 3. 78 ± 1. 87 mm2）. Malondialdehyde level was significantly lower in the TAD-administered group（ 8. 99 ± 0. 21 nmol / g） than in the I / R group（ 12. 22 ± 0. 55 nmol / g）（ P 0. 05）. Histopathologically,TAD-administered group provided significant morphological improvement compared to the I / R group. Conclusion We concluded that TAD prevented I / R-induced neurotoxicity as shown by obtained biochemical and histopathological findings.

关 键 词：他达拉非 脑缺血-再灌注 丙二醛 大鼠 

分 类 号：R255.2[医药卫生—中医内科学] R743[医药卫生—中医学]