MHC-Ⅱ、MHC-Ⅰ及α-SMA在成年比格犬食管肌层的表达  

Expression of MHC-Ⅱ,MHC-Ⅰand α-SMA in muscular layer of esophagus in adult dog

作　　者：周家梅[1] 梁亮[2] 涂丽莉[3] 朱秀玲[4] 张铭[3] 徐胜春[3] 

机构地区：[1]安徽中医药大学人体解剖学教研室,安徽合肥230038 [2]首都医科大学人体解剖学教研室,北京100069 [3]安徽医科大学人体解剖学教研室,安徽合肥230038 [4]皖南医学院人体解剖教研室,安徽芜湖241002

出　　处：《蚌埠医学院学报》2014年第5期568-570,共3页Journal of Bengbu Medical College

基　　金：安徽省自然科学基金资助项目(070413258x)

摘　　要：目的:探讨健康成年比格犬食管肌层骨骼肌肌球蛋白重链-Ⅱ(MHC-Ⅱ)、骨骼肌肌球蛋白重链-Ⅰ(MHC-Ⅰ)和α-平滑肌肌动蛋白(α-SMA)的表达及空间分布。方法:采用组织学、免疫组织化学等方法对6只健康成年比格犬食管肌层中MHC-Ⅱ、MHC-Ⅰ和α-SMA的表达进行观察。结果:成年比格犬食管肌层主要表达MHC-Ⅱ、MHC-Ⅰ;从食管的头端至尾端,MHC-Ⅱ的表达强度呈渐进性减弱,至食管下段近贲门部肌层仍有较强的表达;MHC-Ⅰ在食管肌层的表达由上往下逐渐加强,至食管下段近贲门部肌层有较强表达;α-SMA在成年比格犬食管肌层中段下份开始有少量表达,表达由上往下逐渐加强,至食管下端,α-SMA的表达由内肌层扩展至外肌层。结论:与人类不同,成年比格犬食管肌层主要由骨骼肌构成。成年比格犬食管肌层全长均表达MHC-Ⅱ和MHC-Ⅰ;α-SMA主要表达在食管肌层下段。Objective： To investigate the expressions and spatial distributions of myosin heavy chain-Ⅱ（ MHC-Ⅱ）,myosin heavy chain-Ⅰ（ MHC-Ⅰ） and α-smooth muscle actin（ α-SMA） in muscular layer of esophagus in the healthy adult dogs. Methods： The expressions of MHC-Ⅱ,MHC-Ⅰ and α-SMA in muscular layer of esophagus in 6 healthy adult dogs were detected by histology and immunohistochemistry. Results： The expressions of MHC-Ⅱ and MHC-Ⅰ in muscular layer of whole esophagus in adult dogs were detected. The expression intensity of MHC-Ⅱ gradually decreased from the head to end of esophagus,which was still strong in cardiac muscle layer around the lower esophagus. The expression intensity of MHC-Ⅰ gradually increased from the head to end of esophagus,which was still strong in cardiac muscle layer around the lower esophagus. The little α-SMA expression began in the muscle layer of the middle-lower esophagus,which gradually increased from up to down of esophagus and extended from the inner muscular layer to outer muscular layer of esophagus. Conclusions： Unlike humans,the esophagus muscular layer of adult dog is mainly made up of skeletal muscle. The muscular layer of the whole esophagus in dog expresses the MHC-Ⅱ and MHC-Ⅰ,the expression of a-SMA mainly locates the muscular layer of lower esophagus.

关 键 词：肌球蛋白 肌动蛋白 比格犬 食管肌层 空间分布 

分 类 号：R593.3[医药卫生—内科学]