异氟醚麻醉对新生大鼠海马炎性反应及远期认知功能的影响  

Effects of isoflurane anesthesia on inflammatory responses and long-term cognitive function in hippocampi of neonatal rats

作　　者：刘婷[1] 王寿平[1] 李玉希[1] 洪玉[1] 周礼生[1] 彭书崚[1] 

机构地区：[1]中山大学孙逸仙纪念医院麻醉科,广州市510120

出　　处：《中华麻醉学杂志》2014年第3期283-285,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金面上项目（30772091）,81271223）;广东省自然科学基金项目（S2012010009065）

摘　　要：目的：评价异氟醚麻醉对新生大鼠海马炎性反应及远期认知功能的影响。方法出生7 d的SD大鼠46只，体重12～17 g ，雌雄不拘，采用随机数字表法分为2组（ n＝23）：对照组（C组）和异氟醚麻醉组（I组）。I组吸入2.5％异氟醚3 min ，随后吸入1.5％异氟醚4 h；C组吸入空气。于吸入异氟醚4 h后随机取8只大鼠处死取海马，采用ELISA法测定IL-6、IL-1β和TNF-α含量；余大鼠3周后行旷场实验和Morris水迷宫实验。结果与C组比较，I组潜伏期延长，中央区停留时间缩短，周边区停留时间延长，总路程减少，海马IL-1β和TNF-α含量升高（ P＜0.05）。结论异氟醚麻醉可导致新生大鼠远期认知功能降低，与促进海马炎性反应有关。Objective To evaluate the effects of isoflurane anesthesia on inflammatory responses and long-term cognitive function in the hippocampi of neonatal rats .Methods Forty-six Sprague-Dawley rats of both sexes , aged 7 days ,weighing 12-17 g ,were randomly divided into 2 groups （n=23 each）：control group （group C） and isoflurane anesthesia group （group I ） .In group I ,the rats were exposed to 2.5% isoflurane for 3 min to induce anesthesia and then exposed to 1.5% isoflurane for 4 h to maintain anesthesia ,while in group C the rats were only exposed to air for 4 h .Eight rats in each group were sacrificed and hippocampi were removed for determination of the levels of interleukin-6 ,interleukin-1βand tumor necrosis factor-α.Open field and Morris water maze tests were carried out three weeks later in the left rats .Results Compared with group C ,the escape latency was significantly prolonged ,the time of staying at the central region was shortened ,the time of staying at the border region was prolonged ,the total distance was reduced , and the contents of interleukin-1β and tumor necrosis factor-αwere increased in group I （ P〈0.05） .Conclusion Isoflurane anesthesia results in decreased cognitive function ,which may be related to promotion of inflammatory responses in the hippocampi of neonatal rats .

关 键 词：异氟醚 婴儿 新生 认知障碍 海马 炎症 

分 类 号：R614[医药卫生—麻醉学]