活化蛋白-1在电针上调内毒素休克兔肺组织 HO-1表达中的作用  

Role of activator protein-1 in electro-acupuncture-induced up-regulation of heme oxygenase-1 expression in lung tissues in a rabbit model of endotoxic shock

作　　者：宫丽荣[1] 余剑波[1] 徐妍[1] 曹新顺[1] 史佳[2] 张桂诚[2] 

机构地区：[1]天津市南开医院麻醉科,300100 [2]天津医科大学研究生院

出　　处：《中华麻醉学杂志》2014年第3期348-352,共5页Chinese Journal of Anesthesiology

基　　金：天津市自然科学基金（11JCYBJC11000）;天津市科技支撑重点项目（12ZCZDSY03300）

摘　　要：目的：评价活化蛋白-1（AP-1）在电针上调内毒素休克兔肺组织血红素加氧酶-1（HO-1）表达中的作用。方法健康雄性新西兰大白兔70只，体重1.5～2.0 kg ，2月龄，采用随机数字表法，将其分为7组（ n＝10）：正常对照组（C组）、内毒素休克诱发急性肺损伤组（ALI组）、电针＋ALI组（EL组）、非穴位＋电针＋ALI组（NEL组）、电针＋ALI＋姜黄素组（ELC组）、AP-1抑制剂姜黄素组（Cur组）和二甲基亚砜组（D组）。EL组和ELC组电针刺激双侧足三里及肺俞穴，疏密波，频率15 Hz ，刺激强度以兔出现轻微肌颤为宜，15 min/次，1次/d ，连续5 d ，留针1 h后出针；NEL组以相同电针参数刺激足三里及肺俞穴旁开0.5 cm处。电针刺激5 d后，Cur组及ELC组经耳缘静脉注射姜黄素25 mg/kg （溶于0.5ml0.1％二甲基亚砜），D组静脉注射0.1％二甲基亚砜0.5ml，其余组给予等容量的生理盐水。给药后30 min ALI组、EL组、NEL组及ELC组经耳缘静脉缓慢注射LPS 5 mg/kg （溶于2 ml生理盐水），其余3组给予等容量的生理盐水。给予LPS或生理盐水后6 h时处死取肺组织行病理学评分，计算肺湿/干重比值（W/D比值），检测肺组织MDA含量及SOD活性，采用Western blot法测定HO-1及c-Jun表达水平，采用荧光定量PCR法测定HO-1 mRNA及c-Jun mRNA表达水平。结果与C组比较，ALI组、EL组、NEL组及ELC组肺组织病理学评分、W/D比值和MDA含量升高，SOD活性降低，ALI组、EL组及NEL组HO-1 mRNA、HO-1、c-Jun mRNA和c-Jun表达上调（ P＜0.05），ELC组c-Jun mRNA和c-Jun表达水平差异无统计学意义（ P＞0.05），Cur组及D组上述各指标差异无统计学意义（ P＞0.05）。与ALI组比较，EL组及ELC组肺组织病理学评分、W/D比值和MDA含量降低，SOD活性升高，HO-1 mRNA和HO-1表达上调，EL组c-Jun mRNA和c-Jun表达上调，ELC组c-Jun mRNA和c-Jun表达下调（ P＜0.05）， NEL组上述各指标Objective To evaluate the role of activator protein-1 （AP-1 ） in electro-acupuncture （EA ）-induced up-regulation of heme oxygenase-1 （HO-1 ） expression in lung tissues in a rabbit model of endotoxic shock .Methods Seventy healthy male New Zealand white rabbits ,aged 2 months ,weighing 1.5-2.0 kg ,were randomly divided into 7 groups （ n=10 each ） using a random number table ：normal control group （group C ） , endotoxic shock-induced acute lung injury （ALI ） group （group ALI ） , EA ＋ ALI group （group EL ） , non-acupoint＋EA＋ ALI group （group NEL ） , curcumin （HO-1 inhibitor ） group （group Cur ） , dimethyl sulfoxide group （group D ） ,and EA＋ALI＋curcumin group （group ELC ） .Bilateral 15 min EA stimulation of Zusanli and＆nbsp;Feishu （according to atlas of animals acupoints ） was performed （frequency 15Hz ） once a day for 5 consecutive days before lipopolysaccharide （LPS ） administration in EL and ELC groups ,while in group NEL ,EA stimulation was performed at non-acupoints located 0.5 cm lateral to Zusanli and Feishu acupoints with the same parameters . The animals were anesthetized with urethane and tracheostomized .The animals kept spontaneous breathing .Right internal carotid artery was cannulated for blood pressure monitoring . Ear vein was cannulated for drug administration .At 5 days after EA stimulation ,curcumin 25 mg/kg （in 0.5 ml of 0.1% dimethyl sulfoxide ） was injected in Cur and ELC groups ,0.1% dimethyl sulfoxide 0.5 ml was injected in D group ,and the equal volume of normal saline was given in the other groups .LPS 5 mg/kg （in 2 ml of 0.9% normal saline ） was injected intravenously at 30 min after administration in ALI ,EL ,NEL and ELC groups ,while the equal volume of normal saline was given in the other three groups .Endotoxic shock was confirmed by decrease in mean arterial pressure to 75% of the baseline value within 2 h after LPS injection .Blood samples were collected from the common carotid artery at 6 h after LPS

关 键 词：转录因子AP-1 电刺激疗法 休克 脓毒性 肺 血红素加氧酶-1 

分 类 号：R459.7[医药卫生—急诊医学]