重组人源性白介素-2对耐多药结核病人外周血CD4^＋CD25^＋Foxp3^＋T细胞及TGF-β的影响  

Effect of Recombinant Human IL-2 on the Quantity of the Peripheral Blood CD4^＋CD25^＋Foxp3^＋T Cells and Transforming Growth Factor Beta in Multidrug-resistant Tuberculosis Patients

作　　者：徐传财[1] 黄建安[1] 

机构地区：[1]苏州大学附属第一医院呼吸与危重症医学科,江苏苏州215006

出　　处：《中国血液流变学杂志》2013年第4期635-637,656,共4页Chinese Journal of Hemorheology

摘　　要：目的：探讨重组人源性白介素-2（recombinant human IL-2，rhIL-2）对耐多药结核（multidrug-resistant TB， MDR-TB）病人外周血CD4^＋CD25^＋Foxp3^＋T细胞及转化生长因子-β（transforming growth factor-β，TGF-β）含量的影响。方法选择MDR-TB病人40例，20例化疗，20例化疗＋rhIL-2皮下注射，流式细胞仪检测病人外周血CD4^＋CD25^＋Foxp3^＋T细胞含量，ELISA试剂盒检测外周血TGF-β的表达。结果 MDR-TB病人外周血CD4^＋CD25^＋Foxp3^＋T细胞与TGF-β含量显著相关，治疗后化疗＋rhIL-2组外周血CD4^＋CD25^＋Foxp3^＋T细胞及TGF-β含量分别下降（1.55±0.29）%和（18.97±2.11）%，与化疗组的（0.83±0.13）%和（10.98±1.04）%相比差异有统计学意义（P＜0.05）。结论 CD4^＋CD25^＋Foxp3^＋T细胞及TGF-β在MDR-TB的发病中起重要作用，应用rhIL-2能够降低外周血CD4^＋CD25^＋Foxp3^＋T细胞及TGF-β的含量。Objective To investigate the effect of recombinant human IL-2 on the quantity of the peripheral blood CD4^＋CD25^＋Foxp3^＋T cells and TGF-β in multidrug-resistant tuberculosis patients. Methods We selected multidrug-resistant TB patients, Flow cytometry was used to determine the quantity of CD4^＋CD25^＋Foxp3^＋T cells in the peripheral blood. The level of TGF-β was detected by ELISA. Results The TGF-β level and the CD4^＋CD25^＋Foxp3^＋T cells/CD4＋cells ratio in the peripheral blood of patients with MDR-TB was signiifcantly correlated （r=0.80, P〈0.01）. The quantity of CD4^＋CD25^＋Foxp3^＋T cells and the level of TGF-βin the peripheral blood of patients of chemotherapy plus recombinant human IL-2 group signiifcantly decreased after the treatment when compared with that of chemotherapy group. Conclusion CD4^＋CD25^＋Foxp3^＋T cell and TGF-βplay an im-portant role in the pathogenesis of multidrug-resistant TB. Recombinant human IL-2 has an effect on the quantity of the peripheral blood CD4^＋CD25^＋Foxp3^＋T cells and TGF-βin multidrug-resistant tuberculosis patients.

关 键 词：Foxp3 耐多药结核 白介素-2 TGF-Β 

分 类 号：R521[医药卫生—内科学]