氯胺酮对重度烧伤患者辅助T细胞分化的影响  

Effects of Ketamine on Differentiation of Severe Burn Patients Helper T Cells

作　　者：顾成永[1] 徐毅明[1] 费海涛[2] 吴亚谋[1] 

机构地区：[1]苏州市立医院北区麻醉科,江苏苏州215008 [2]解放军第101医院麻醉科,江苏无锡214044

出　　处：《中国血液流变学杂志》2013年第4期654-656,共3页Chinese Journal of Hemorheology

摘　　要：目的：观察不同浓度氯胺酮对重度烧伤患者静脉血辅助T细胞（Th细胞）分化的影响。方法采集10例重度烧伤患者外周静脉血20 mL。进行全血和外周血单个核细胞（PBMCs）培养。每一份血样均用于下列各组研究：空白对照组（C组）、氯胺酮50μg/mL组（K1组），氯胺酮150μg/mL组（K2组）。淋巴细胞经过PHA刺激48 h后，监测淋巴细胞中Th1、Th2和Th17细胞亚群及其Th1/Th2比值。结果与C组比较，K1组和K2组的Th1、Th2和Th17细胞亚群的数量没有减少（P＞0.05），且Th1/Th2比值也没有明显变化（P＞0.05）。结论氯胺酮对重度烧伤患者的辅助T细胞分化没有明显的抑制，可以安全使用在烧伤手术中。Objective To investigate the effects of ketamine on severe burn patients T helper （Th） cells dif-ferentiation. Methods Ten severe burn patients were enrolled in the study. In each patient 20 mL of blood was taken from peripheral vein and divided into 3 groups：control group （0.9%NaCl, group C）, 2 ketamine groups （50, 150μg·mL^-1, group K1 and group K2）. Whole blood and mononuclear cells from peripheral blood （PBMCs） were incubated in the presence of 0.9%NaCl or different concentrations of ketamine for 24 h. Then human T helper cell were stimulated with PHA for 48 h. The subsets of Th1, Th2 and Th17 cells were analyzed with lfowcytom-etry. Results Compared with group C, the number of Th1, Th2 and Th17 were no different （P〉0.05）. The Th1/Th2 ratio was no different （P〉0.05）. Conclusion Ketamine on severe burn patients T helper cells differentiation was not inhibited. It can be safely used.

关 键 词：烧伤 氯胺酮 辅助T细胞 免疫 

分 类 号：R614.1[医药卫生—麻醉学]