葡萄蔓基质中九种食药用菌的筛选及相关酶学检测  

Mycelial Growth Rates and Cellulase Activity of Nine Mushroom Species Cultivated on Substrates Containing Vine Trimmings

作　　者：刘敏[1] 马旭光[1] 苏岩[1] 王莎[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《食用菌学报》2013年第4期27-30,共4页Acta Edulis Fungi

基　　金：河北省科技支撑项目(编号:11230910D)的子课题资助

摘　　要：以菌丝生长速度、羧甲基纤维素酶活性和滤纸酶活性为指标,在20%、40%、60%、80%、90%葡萄蔓取代杂木屑的培养料中对九种食药用菌进行生长筛选,结果表明,韩芝HG(Ganoderma lucidum)在各个比例的配方中对纤维素的酶解能力最高,菌丝生长最快。在初筛基础上子实体栽培实验结果表明,韩芝在60%葡萄蔓、30%木屑、9%麸皮、1%生石灰培养料中产量和生物转化率都高于常规培养料。Mycelial growth rates and extracellular cellulase activities（CMCase and FPase）of nine species of edible and medicinal mushrooms were determined during cultivation on substrates supplemented with 20-90%grape vine trimmings.Highest growth rates and enzyme activities were recorded with Ganoderma lucidum（strain HG）when cultivated on a substrate containing 60% vine trimmings,30% sawdust,9% wheat bran and 1%lime.Under these conditions,fruit body yields and biological efficiency values were similar to those recorded with a control substrate without vine trimmings and containing 90%sawdust.

关 键 词：葡萄蔓 韩芝 菌丝生长速度 胞外纤维素酶活性 

分 类 号：S646[农业科学—蔬菜学]