捏脊疗法对颈椎病模型大鼠纤维环细胞影响的实验研究  

Experimental study on effect of chiropractic therapy on fiber ring cell in cervical spondylosis rats

作　　者：廖军[1] 谢巧瑜[1] 张乐[1] 郑佳璇[1] 柯玫瑰[2] 黄萍萍[1] 

机构地区：[1]福建中医药大学针灸学院,福州350122 [2]厦门市第二医院海沧分院康复理疗科,厦门361026

出　　处：《中国中医基础医学杂志》2014年第5期611-614,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(81273836&81001554)

摘　　要：目的:探讨捏脊对大鼠动静力失衡颈椎病模型纤维环细胞的影响。方法:将SD大鼠按随机数字表法分为空白组、模型组、捏脊组和莫比可组。治疗1月后取材,运用免疫组化测基质金属蛋白酶-l(MMP-1)、基质金属蛋白酶-3(MMP-3)、细胞外信号调节蛋白激酶1/2(ERK1/2)、蛋白激酶B(Akt)表达的变化。结果:与空白组比较,模型组MMP-1、MMP-3阳性表达明显升高(P<0.05);与模型组比较,捏脊组MMP-1、MMP-3显著降低(P<0.05);与空白组比较,模型组Akt、ERK1/2明显降低(P<0.05);与模型组比较,捏脊组Akt、ERK1/2阳性细胞数升高,差异有统计学意义(P<0.05)。结论:捏脊疗法可以下调退变椎间盘纤维环细胞MMP-1、MMP-3及上调纤维环细胞中Akt、ERK1/2蛋白表达,从而改善颈椎间盘退变。Objective ： To explore the impacts of spine pinching on the annulus fibrosus cells in the rat model of cervical spondylosis with dynamic and static imbalance force. Methods：The rats were randomly divided into 4 groups, including a pinching spine group, a meloxicam group, a model group and a blank group. After treatment of 1 month, the expression of matrix metalloproteinase-1 （ MMP-1 ） , matrix metalloproteinase-3 （ MMP-3 ）, extracellular signal regulated protein kinase （ERK1/2）, protein kinase B （Akt） proteins in the annulus fibrosus cells were detected by immunohistochemical. Results：The expression of MMP-1, MMP-3 proteins in the model group were significantly increased than the blank group （P 〈 0.05 ）, and after chiropractics treatment, MMP-1, MMP-3 proteins markedly decreased than the model group （P 〈 0. 05）. Compared with the blank group, the expression of Akt, ERK1 /2 proteins in the model group were significantly decreased （P 〈 0.05） , and compared with the model group, the expression of Akt, ERK1 /2 proteins in the pinching spine group were significantly increased （P 〈 0.05 ）. Conclusion ： Spine pinching can down-regulate expression of MMP-1, MMP-3 proteins and up-regulate expression of Akt, ERK1 /2 proteins, so as to improve the cervical intervertebral disc degeneration.

关 键 词：颈椎病 捏脊 纤维环细胞 基质金属蛋白酶-1 基质金属蛋白酶-3 蛋白激酶B 细胞外信号调节蛋白激酶 

分 类 号：R681.55[医药卫生—骨科学]