雌二醇对NZB/wF1狼疮鼠脾脏树突状细胞雌激素受体表达的影响  

Effects of 17β-estrodiol on the expression of estrogen receptor in spleen dendritic cells of lupus model-NZB/wF1 mice

作　　者：姜波[1] 杨希[1] 孙凌云[1] 侯亚义[2] 

机构地区：[1]南京大学医学院附属南京鼓楼医院风湿免疫科,210008 [2]南京大学医学院分子免疫实验室

出　　处：《中华临床医师杂志（电子版）》2013年第11期101-103,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金(81102257);南京市卫生青年人才培养工程(2012-2015)

摘　　要：目的通过检测系统性红斑狼疮(SLE)模型鼠脾脏树突状细胞(DC)中雌激素受体(ER)的表达及对体外雌激素的反应性是否异常,探索雌激素调控DC参与SLE发病的机制。方法红细胞裂解法收集SLE模型鼠———NZB/wF1雌鼠及BALB/c雌鼠的脾脏单个核细胞,抗小鼠CD11c单抗标记磁珠纯化DC,以无酚红RPMI-1640及葡聚糖-活性炭处理的新生牛血清体外培养DC,加入不同浓度10-8mol/L,10-7mol/L,10-6mol/L的雌二醇(E2)体外处理24 h,RT-PCR检测DC胞内ER的mRNA水平。结果两种小鼠脾DC均表达ERα,但不表达ERβ;NZB/wF1雌鼠脾DC的ERα基础表达显著高于同周龄的BALB/c雌鼠(0 mol/L组P<0.05);低浓度10-8mol/L E2显著增加两种鼠脾DC的ERα表达(NZB/wF1鼠P<0.05,BALB/c鼠P<0.05),中浓度10-7mol/L(NZB/wF1鼠P<0.05,BALB/c鼠P<0.05)和高浓度10-6mol/L(NZB/wF1鼠P<0.05,BALB/c鼠P<0.05)E2显著降低两种鼠脾DC的ERα表达。结论 ER表达在狼疮鼠中存在明显异常,不同剂量雌激素对其受体调控作用不同,提示雌激素可能通过调控DC的ER参与SLE发病。Objective To research the mechanism how estrogen abnormally modulates dendritic cells（DC） to involve in systemic lupus erythematosus（SLE） by investigating the expression of estrogen receptor（ER） in spleen DCs of SLE model mice and the reaction of ER to estrogen in vitro.Methods Harvest the spleen mononuclear cells of female NZB/wF1 and BALB/c mice by lysing erythrocytes.Monoclonal anti-mouse CD11c antibody-labeled bead was used to purify DCs from mononuclear cells.DC were cultured in phenol red-free RPMI1640 with 10％ charcoaldextran treated fetal bovine serum and different concentration：10-8 mol/L,10-7 mol/L,10-6 mol/L of 17β-estrodiol （E2） for 24 hours.RT-PCR was used to detect the mRNA level of ER in E2-stimulated lymphocytes.Results The spleen DC of female NZB/wF1 and BALB/c mice both express the mRNA of ERα,but not ERβ.The expression of ERαin NZB/wF1 spleen DC was significantly higher than same week-old BALB/c mice （0 mol/L P ＜ 0.05）.Low （10-8 mol/L）concentration of E2 obviously promoted the expression of ERα in the two kinds of mice （NZB/wF1 mice P ＜ 0.05,BALB/c mice P ＜ 0.05）.Middle （10-7 mol/L,NZB/wF1 mice P ＜ 0.05,BALB/c mice P ＜ 0.05）and high （10-6 mol/L,NZB/wF1 mice P ＜ 0.05,BALB/c mice P ＜ 0.05） concentration of E2 decreased the expression of ERα.Conclusion The expression of estrogen receptor is obviously abnormal in lupus model mice.ER reactions to various concentration of estrogen in vitro are different.It suggests that estrogen may involve in the pathogenesis of SLE by modulating estrogen receptors in DC.

关 键 词：红斑狼疮 系统性 雌二醇 树突细胞 受体 雌激素 

分 类 号：R593.241[医药卫生—内科学]